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目的:研究川芎嗪对辐射所致小鼠肾脏氧化损伤的预防和治疗作用。方法:采用60Co-γ射线5 Gy全身单次照射小鼠造模,在照射前和照射后分别于每天腹腔注射川芎嗪130 mg/kg,连续给药10 d,进行预防和治疗,并设对照组,观察肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)及总抗氧化力(T-AOC)的变化。结果:与阴性对照组比较,照射可显著增加肾组织中MDA的含量(P<0.05),降低SOD、CAT的活性(P<0.05),升高GSH-Px活性(P<0.05),降低GSH含量(P<0.05),使肾组织T-AOC下降(P<0.05),。与照射组比较,给予川芎嗪预防和治疗后,均可降低肾组织MDA含量(P<0.05),升高肾组织T-AOC(P<0.05),且治疗组优于预防组,与阴性对照组无显著性差异。同时,预防组可使SOD活性和GSH含量升高(P<0.05),治疗组可使SOD和CAT活性增高(P<0.05),但均对GSH-Px活性无显著影响(P>0.05)。结论:川芎嗪具有很好的抗氧化作用,无论预防和治疗均可降低辐射所致小鼠肾脏的氧化应激损伤,并且治疗效果优于预防效果。
Objective: To study the preventive and therapeutic effects of ligustrazine on renal oxidative damage induced by radiation in mice. Methods: A single-dose 5 Gy whole body irradiation with 60Co-γ-rays was used to establish the model. Ligustrazine (130 mg / kg) was intraperitoneally injected before and after irradiation for 10 consecutive days for prevention and treatment. The changes of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GSH -Px) and total antioxidant capacity (T-AOC) changes. Results: Compared with the negative control group, irradiation could significantly increase the content of MDA in renal tissue (P <0.05), decrease the activity of SOD and CAT (P <0.05), increase the activity of GSH-Px (P <0.05), the T-AOC in renal tissue decreased (P <0.05). Compared with the irradiation group, administration of ligustrazine could reduce the content of MDA in renal tissue (P <0.05) and increase the T-AOC in renal tissue (P <0.05), and the treatment group was better than the prevention group and the negative control group No significant difference between groups. At the same time, the activity of SOD and the content of GSH in the prophylaxis group increased (P <0.05). The activity of SOD and CAT in the treatment group increased (P <0.05), but both had no significant effect on the activity of GSH-Px (P> 0.05). CONCLUSION: Tetramethylpyrazine has a good anti-oxidant effect. Both prevention and treatment can reduce the oxidative stress injury of the kidney caused by radiation in mice, and the treatment effect is better than the preventive effect.