2012—2015年广东省珠三角某市公共场所嗜肺军团菌污染状况及血清型

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目的了解2013—2015年广东省珠三角某市公共场所嗜肺军团菌污染情况。方法 2012—2015年随机采集495家公共场所冷却水、冷凝水、淋浴水、自来水共995份,水样依照WS 394 2012《公共场所集中空调通风系统卫生规范》检测嗜肺军团菌及其菌型。结果嗜肺军团菌阳性率冷却水为8.21%,冷凝水4.78%,淋浴水为5.77%,15份自来水未检出嗜肺军团菌。嗜肺军团菌阳性率在购物场所为3.3%)、沐浴场所为5.31%、住宿场所为13.0%,文化娱乐场所为5.68%);场所间差异有统计学意义(χ~2=25.182,P<0.01)。将分离出的72株嗜肺军团菌做菌型鉴定,分型结果以Lp1型为主,占58.4%,Lp7型占22.5%,其他菌型占19.1%。结论 2012—2015年广东省珠三角某市公共场所水系统冷却水、冷凝水和淋浴水均存在一定程度的嗜肺军团菌污染,血清型呈多样性,以Lp1型和Lp7型为主,应加强住宿场所空调系统的清洗消毒。 Objective To understand the contamination of Legionella pneumophila in public places in a certain city of PRD in Guangdong Province from 2013 to 2015. Methods A total of 995 cooling water, condensate water, shower water and tap water were collected randomly from 495 public places in 2012-2015. The water samples were tested for Legionella pneumophila and its bacterium according to WS 394 2012 Hygienic Standard for Central Air Conditioning and Ventilation System of Public Places . Results Legionella pneumophila positive rate of cooling water was 8.21%, condensate 4.78%, shower water was 5.77%, 15 parts of tap water did not detect Legionella pneumophila. The positive rate of Legionella pneumophila was 3.3% in shopping places, 5.31% in bathing places, 13.0% in places of accommodation and 5.68% in places of cultural entertainment). There was significant difference between sites (χ ~ 2 = 25.182, P < 0.01). The 72 strains of Legionella pneumophila isolated were identified as bacterias. The typing results were mainly Lp1 type, accounting for 58.4%, Lp7 type accounting for 22.5% and other strains accounting for 19.1%. Conclusions There is a certain degree of Legionella pneumophila contamination in the cooling water, condensate and shower water of public places in the Pearl River Delta of Guangdong Province from 2012 to 2015. The serotypes are diverse, mainly Lp1 and Lp7 To strengthen the cleaning and disinfection of air-conditioning systems in accommodation establishments.
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