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寻找内毒素和培养的内皮细胞直接结合的证据及对胞内连接蛋白α－Ｃａｔｅｎｉｎ的影响，观察内毒素对内皮细胞的损伤特性。方法：（１）荧光标记的内毒素（２μｍ／ｍｌ）和培养的内皮细胞在无血清下共同孵育后用荧光分光光度计进行检测；（２）内毒素（２μｇ／ｍｌ）刺激无血清培养的内皮细胞后用ＥＬＩＳＡ测定内皮细胞胞内连接蛋白α－Ｃａｔｅｎｉｎ表达的变化。结果：内皮细胞和内毒素孵育１０、２０、４０和６０分后荧光强度分别为对照组的１．８７、３．９７、３．４１（Ｐ＜０．０１）和２．０２（Ｐ＜０．０５）倍；胞内连接蛋白的表达各时相点和对照组比均明显降低（ Ｐ＜０． ０５和＜０． ０１）。结论：内毒素在无血清情况下可以和内皮细胞结合，同时可以影响胞内连接蛋白α－Ｃａｔｅｎｉｎ的表达状态，并不依赖是否有血清的存在。
Looking for evidence of direct binding of endotoxin to cultured endothelial cells and the effect on the intracellular connexin α-Catenin, and observing the damage characteristics of endotoxin to endothelial cells. Methods: (1) Fluorescence labeled endotoxin (2μm / ml) and cultured endothelial cells were incubated in serum-free medium and detected by fluorescence spectrophotometer; (2) Endotoxin (2μg / ml) Endothelial cells were assayed by ELISA for the change of intracellular connexin α-Catenin expression. Results: The fluorescence intensities of endothelial cells and endotoxin incubated for 10, 20, 40 and 60 minutes were 1.87, 3.97 and 3.41 (P <0.01) and 2.02 .05) fold; intracellular connexin expression at each time point and the control group were significantly lower (P <0.05 and <0.01). CONCLUSION: Endotoxin can bind endothelial cells in serum-free condition and affect the expression of α-Catenin, which is not dependent on the presence or absence of serum.