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目的了解2014年-2015年龙岩市判定为其他EV的HFMD患者病原构成情况,为龙岩市的HFMD防控提供科学依据。方法对580例其他EV HFMD患者的标本用RT-PCR方法检测Cox A6、Cox A10和Echo30,仍无法分型的样本,经RD细胞分离培养后,再次对培养物进行特异性核酸检测。结果 580份样本中检出EV阳性580份,阳性率为100.00%。Cox A6检出率最高,达61.38%,2015年高达71.55%。HFMD其他EV样本分布均以Cox A6为主,阳性检出率高峰均在10月。阳性检出人群主要集中在3岁及以下儿童,占检测总样本数的91.03%,其中2岁~组所占比例最高,为39.48%,不同年龄组阳性检出率差异有统计学意义(χ~2=416.88,P<0.05)。结论 2014年-2015年引起龙岩市HFMD的其他EV中,病原型别众多,尤其是Cox A6已成为本市HFMD的主要病原体,因此建议将其纳入HFMD的日常监测,并在其多发季节加大防控力度。
Objective To understand the etiopathogenisis of HFMD patients in Longyan city from 2014 to 2015 and to provide a scientific basis for the prevention and control of HFMD in Longyan City. Methods Cox A6, Cox A10 and Echo30 were detected by RT-PCR in 580 cases of other patients with EV HFMD. The samples still unable to be typed were detected by RT-PCR. After RD cells were separated and cultured, specific nucleic acids were detected again. Results Among the 580 samples, 580 were positive for EV, the positive rate was 100.00%. Cox A6 detected the highest rate of 61.38% in 2015 up to 71.55%. The distribution of other EV samples in HFMD was mainly Cox A6, and the positive detection rate was all in October. Positive detection of the crowd mainly concentrated in children 3 years of age and below, accounting for 91.03% of the total number of samples tested, of which the highest proportion of 2-year-old group was 39.48%, positive detection rate of different age groups were statistically significant (χ ~ 2 = 416.88, P <0.05). Conclusions Among the other EVs that caused HFMD in Longyan from 2014 to 2015, there were many pathogen types, especially Cox A6, which has become the major causative agent of HFMD in our municipality and is therefore recommended for routine monitoring of HFMD and increased in its multiple seasons Prevention and control.