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目的制备高温气体吸入致豚鼠急性肺损伤(ALI)动物模型。方法选择20只雄性豚鼠随机分为2组,每组10只,其中健康豚鼠为对照组、急性肺损伤豚鼠为实验组。实验组吸入150℃高温气体制作豚鼠ALI动物模型,然后处死2组豚鼠并取豚鼠右肺的大体标本,称重计算豚鼠肺湿/干重比(W/D),选取2组豚鼠左上肺组织进行HE染色,利用光学显微镜观察肺组织病理变化情况,及时记录其病理变化的结果;对豚鼠动脉血液进行血气分析并比较。结果实验组豚鼠肺组织病理结构呈现明显的急性炎症性改变;实验组豚鼠肺W/D比值显著高于对照组[(5.72±0.31)vs(4.88±0.11),P<0.05];实验组豚鼠pH、PaO_2显著低于对照组[(7.02±0.12)vs(7.38±0.04),(98.80±62.60)mm Hg vs(177.30±24.70)mm Hg,P<0.05],而PaCO_2显著高于对照组[(74.70±23.23)mm Hg vs(31.10±8.77)mm Hg,P<0.05]。结论成功制作豚鼠ALI实验动物模型。该模型达到ALI要求,仿真性能高、重复性好、稳定性高,为今后研究高温气体吸入致ALI的临床及基础研究提供了可靠的动物模型。
Objective To prepare animal model of acute lung injury (ALI) induced by high temperature gas inhalation in guinea pigs. Methods Twenty male guinea pigs were randomly divided into two groups (n = 10 in each group). The healthy guinea pigs served as the control group and the acute lung injury guinea pigs served as the experimental group. The experimental group inhaled high temperature gas at 150 ℃ to make guinea pig ALI animal model, and then two groups of guinea pigs were sacrificed and the guinea pigs were taken from the right lung. The wet / dry weight ratio (W / D) of guinea pigs was weighed and the left upper lung tissue HE staining, the use of optical microscopy lung pathological changes, and timely records of the results of its pathological changes; blood gas in guinea pig arterial blood analysis and comparison. Results The pathological changes of lung tissue in experimental group showed obvious acute inflammatory changes. The lung W / D ratio in experimental group was significantly higher than that in control group [(5.72 ± 0.31) vs (4.88 ± 0.11), P <0.05] pH and PaO_2 were significantly lower than those in the control group [(7.02 ± 0.12) vs (7.38 ± 0.04), (98.80 ± 62.60) mm Hg vs (177.30 ± 24.70) mm Hg, P <0.05] (74.70 ± 23.23) mm Hg vs (31.10 ± 8.77) mm Hg, P <0.05]. Conclusion The experimental model of ALI in guinea pigs was successfully established. The model meets the ALI requirements and has high simulation performance, good repeatability and high stability, and provides a reliable animal model for clinical and basic research on ALI induced by high temperature gas inhalation in the future.