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目的验证多重荧光定量RT-PCR方法用于麻疹病原学诊断的准确性,探讨其在麻疹快速检测方面的适用性。方法对吉林省2013年的149例麻疹疑似病例咽拭子标本,进行多重荧光定量RT-PCR、双重荧光定量RT-PCR实验,对52份获得麻疹病毒序列的标本进行多重荧光定量RT-PCR和序列分析实验,并将2组实验结果分别进行对比分析。结果多重荧光定量RT-PCR检测结果与麻疹/风疹双重荧光定量RT-PCR检测结果一致,均得到140份麻疹阳性标本、9份阴性标本,一致率为100%;多重荧光定量RT-PCR检测结果与序列分析结果均显示JL/M-13-3号标本为疫苗株A基因型,其他51份标本为野毒株H基因型,结果吻合率为100%。结论多重荧光定量RT-PCR是一种快速、灵敏、准确的实验方法,适用于快速诊断麻疹病例、及时鉴别麻疹H基因型野病毒和A基因型疫苗株引起的病例。
Objective To verify the accuracy of multiple fluorescence quantitative RT-PCR in the diagnosis of measles and to explore its applicability in the rapid detection of measles. Methods Thirty-nine cases of measles suspected throat swab specimens from Jilin Province in 2013 were subjected to multiplex fluorescence quantitative RT-PCR and double fluorescence quantitative RT-PCR. Fifty-two specimens of measles virus were detected by multiplex fluorescence quantitative RT-PCR and real- Sequence analysis experiments, and two groups of experimental results were compared. Results The results of multiplex fluorescence quantitative RT-PCR were consistent with those of measles / rubella double-quantitative RT-PCR, and all 140 measles positive samples and 9 negative samples were obtained. The coincidence rate was 100%. The results of multiplex fluorescence quantitative RT-PCR The result of sequence analysis showed that JL / M-13-3 was genotype A of vaccine strain, and the other 51 genotypes were wild type H genotype. The coincidence rate was 100%. Conclusion Multiplex fluorescence quantitative RT-PCR is a fast, sensitive and accurate experimental method, which is suitable for rapid diagnosis of measles cases and timely identification of cases caused by the wild type H and wild-type A vaccine strains.