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目的将经典光学理论运用于血栓透射模型 ,使用灰度补偿法三维重建血栓 ,为光透射法研究血栓提供了一种全新的工具。通过对血栓轮廓的精确描绘 ,使得研究者可以利用跨学科手段区别各个局部物理、生理条件的差异。方法用数字摄像机记录长达40min的血栓形成过程 ,缩时摄影每隔10s钟记录一帧。这些数字图片将被用来重建血栓的三维影像 ,其中包括药物作用下以及不同血流情况下的动静脉血栓。我们证明了灰度补偿(Gray -compensate)法对血栓进行三维重建 ,并通过对闭塞的血栓图片处理所得的灰度补偿值Gc与血管内径比较 ,可以计算得出真实厚度和真实体积。该结果与目前通用的光密度单位A.U.保持高度线性说明了该方法的精确性。结果获得血栓的三维影像 ,并初步分析了实验性仓鼠在体血栓形成机理以及抗栓药物药效。发现血栓在尾涡或湍流区存在明显的生长优势 ,从而通过在体实验证实了离体实验的结论。同时也观察到动脉中由于血液流动总体剪切率较高 ,血栓生长基本集中在剪切率相对较低的区域 ,而静脉血栓由于血液流动总体剪切率较低 ,即使在剪切率相对较高的区域仍有明显的生长。结论这种三维重建不但提供了血栓的三维轮廓 ,而且还提供了血栓各局部生长速度这一重要参数。随着研究的深入 ,这种新方法将会带给研究?
Objective To apply classical optics theory to thrombus transmission model and to reconstruct thrombus by gray-scale compensation method, which provides a new tool for studying light thrombus by light transmission method. By accurately depicting the thrombus profile, researchers can make use of interdisciplinary approaches to differentiate individual local physical and physiological differences. Methods A digital camera was used to record thrombus formation up to 40 minutes. Shrink time recording recorded one frame every 10 seconds. These digital images will be used to reconstruct three-dimensional images of thrombi, including arteriovenous thrombosis under the influence of drugs and different blood flow conditions. We demonstrate that the Gray-compensating method can reconstruct the thrombus three-dimensionally and calculate the true thickness and the real volume by comparing the Gc value of the occluded thrombus image with the inner diameter of the blood vessel. The accuracy of the method is demonstrated by the fact that the results are highly linear with the currently used optical density unit A.U. Results Three-dimensional images of thrombus were obtained. The mechanism of thrombosis in vivo and the efficacy of antithrombotic drugs were also analyzed. The thrombus was found to have obvious growth advantage in the caudal vortex or turbulence area, and the conclusion of the in vitro experiment was confirmed by in vivo experiments. Also observed in the arteries due to the overall high shear rate of blood flow, thrombus growth is basically concentrated in the shear rate is relatively low region, and venous thrombosis due to the lower overall blood flow shear rate, even in the shear rate is relatively High areas still have significant growth. Conclusion This three-dimensional reconstruction not only provides a three-dimensional profile of the thrombus but also provides an important parameter for the local growth rate of the thrombus. With the deepening of research, this new method will bring research?