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目的:建立高效液相色谱法测定益心康泰胶囊中大黄素、大黄酚含量。方法:以Hypersil-ODS2C18(4.6mm×250mm,5μm)为分析柱,流动相为甲醇-0.1%磷酸(80∶20),检测波长为254nm,流速为1mL·min-1,柱温20℃,进样量10μL。结果:大黄素在0.04～0.80mg·L-1线性关系良好(r=1.0000),平均回收率为99.5%(RSD1.81%);大黄酚在0.1102～2.204mg·L-1线性关系良好(r=1.0000),平均回收率为99.1%(RSD1.80%)。结论:样品处理方法简便,灵敏度高,结果准确。
Objective: To establish a method for the determination of emodin and chrysophanol in Yixinkangtai capsule by high performance liquid chromatography. METHODS: The analytical column was Hypersil-ODS2C18 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-0.1% phosphoric acid (80:20), the detection wavelength was 254 nm, the flow rate was 1 mL · min- Injection volume 10μL. Results: The emodin showed good linearity (r = 1.0000) at 0.04 ~ 0.80mg · L-1 and the average recovery was 99.5% (RSD 1.81%). Chrysophanol had a good linear relationship at 0.1102 ~ 2.204mg · L-1 r = 1.0000) with an average recovery of 99.1% (RSD 1.80%). Conclusion: The sample processing method is simple, high sensitivity and accurate results.