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目的研究炎性微环境对人牙周膜干细胞(孕阅蕴杂悦泽)和骨髓间充质干细胞(月MM杂悦泽)骨向分化的效用。方法培养正常的牙周膜干细胞，在培养基中加入栽晕云-α制作炎性微环境。在炎性微环境下培养牙周膜干细胞和骨髓间充质干细胞，再对两种干细胞碱性磷酸酶染色、增殖能力和骨向分化基因表达进行检测。结果炎性微环境下，牙周膜干细胞相对于第一组正常牙周膜干细胞组孕阅蕴杂悦泽碱性磷酸酶染色减弱，而月MM杂悦泽碱性磷酸酶染色相对于正常培养环境下的骨髓间充质干细胞未见明显减弱。而细胞增殖实验显示孕阅蕴杂悦泽增殖能力较月MM杂悦泽强。结论炎症微环境下，两种干细胞骨向分化能力均减弱；在两种干细胞中，月MM杂悦泽骨向分化能力较高、对炎症刺激抵抗较强。“,”Objective To investigate the dif erence of osteoblastic dif erentiation between PDLSCs and BMMSCs under inflamatory microenviroment. Methods PDLSCs were culturedand identified. Cel proliferation and osteogenic dif erentiation ability were tested by MTT and alkaline phosphatase staining.Results Treatment with TNF-a, PDLSCs to osteogenic dif erentiation were inhibted significantly, while in the control group, BMMSCs showed no significant ef icacy under the same condition. Conclusion The result showed BMMSCs have stronger motivation of osteogenic dif erentiation,while PDLSCs are far away behinder.