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目的 研究银杏叶提取物对大鼠坐骨神经损伤后相应脊神经节感觉神经元超微结构的影响。方法 将SD大鼠 36只随机分为银杏叶提取物组和生理盐水组。制作左侧坐骨神经部分切除及反折结扎模型。术后两组分别经腹腔注射银杏叶提取物 (1 0 0mg·kg 1 ·d 1 )和同体积的生理盐水。 2、4、6周取材 ,电镜观察L4 及L5脊神经节感觉神经元超微结构形态变化 ,体视学方法计算并比较两组感觉神经元线粒体、内质网及溶酶体体积密度变化。取L6 神经节 ,光镜下计数并计算神经节感觉神经元存活率。结果 实验组超微结构形态变化明显轻于对照组。术后 2、4、6周神经元线粒体、内质网及溶酶体体积密度值均小于对照组 ,其差异有显著性 (P <0 .0 5)。实验组 2、4及 6周感觉神经元存活率为 85 .58%、82 .45 %和 79.1 1 % ,存活率大于对照组 (P <0 .0 5)。结论 银杏叶提取物 ,对坐骨神经损伤后感觉神经元有一定保护作用
Objective To study the effects of Ginkgo biloba extract on the ultrastructure of sensory neurons in the corresponding spinal ganglia following sciatic nerve injury in rats. Methods 36 SD rats were randomly divided into Ginkgo biloba extract group and saline group. A left sciatic nerve partial resection and reflex ligature model was made. The two groups were injected intraperitoneally with Ginkgo biloba extract (100 mg·kg 1 ·d 1 ) and the same volume of saline. The morphological changes of L4 and L5 spinal ganglia sensory neurons were observed by electron microscopy at 2, 4, and 6 weeks. Stereological methods were used to calculate and compare the changes of mitochondria, endoplasmic reticulum and lysosome volume densities of sensory neurons. The L6 ganglion was taken and counted under light microscope to calculate the survival rate of ganglion sensory neurons. Results The ultrastructural changes of the experimental group were significantly lighter than those of the control group. The volume density of mitochondria, endoplasmic reticulum and lysosome in neurons at 2, 4, and 6 weeks after operation were all significantly smaller than those in the control group (P < 0.05). In the experimental group, the survival rate of sensory neurons at 2, 4, and 6 weeks was 85.58%, 82.45 %, and 79.11%, and the survival rate was greater than that of the control group (P < 0.05). Conclusion Ginkgo biloba extract has a protective effect on sensory neurons after sciatic nerve injury