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RhoGAPs(GTPase activating proteins)通过与Rho GTP酶结合,促使GTP酶水解成Rho GDP无活状态,在生物体中调控着重要的生物学功能。在全基因组上对玉米弯孢叶斑病菌(C.lunata)中RhoGAP基因家族成员进行鉴定,对其序列特征分析。结果表明,玉米弯孢叶斑病菌(C.lunata)有5个RhoGAP基因家族成员,分布在5不同的Scaffold上,其基因结构分析显示,外显子和内含子大小与位置均不一样,表现出复杂的基因结构。序列比对和系统进化分析发现,5个RhoGAP氨基酸序列与其他真菌来源的RhoGAP一样具有特征性保守序列区,与粗糙脉胞菌(N.crassa)和小麦黄斑叶斑病菌(P.tritici-repentis)的RhoGAP蛋白关系密切,其亲缘关系最近。
RhoGAPs (GTPase activating proteins) regulate GTPases to Rho GDP via their binding to Rho GTPases, which regulate important biological functions in the organism. The genome-wide RhoGAP gene family members of C.lunata were identified and their sequence characteristics were analyzed. The results showed that there were 5 members of RhoGAP gene family in C.lunata distributed on 5 different Scaffolds. The gene structure analysis indicated that the size and position of exon and intron were different, Showing complex genetic structure. Sequence alignment and phylogenetic analysis showed that the five RhoGAP amino acid sequences shared the same conserved sequences as RhoGAP derived from other fungi, ) RhoGAP protein is closely related, the closest relationship.