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本文使用健康、雄性Wistar大鼠,在直视下结扎左右锁骨下动脉和颈总动脉,持续结扎时间分别为5、10、20和30min,然后解除两侧颈总动脉结扎使脑血管再通。在实验过程中动态观察脑电图、心电图和眩孔颜色的变化,实验结束后检查血管结扎是否准确,并从升主动脉注射伊文思蓝观察染料在脑内的分布,灌注固定后取脑进行形态学检查。结果发现全部动物血管结扎部位准确,注射染料证实,使用本方法造成的脑缺血和再灌流效果可靠。在血管结扎后的几秒钟到十几秒钟内全部动物的脑电图完全抑制,并在持续结扎的5、10、20和30min内持续不变;结扎5和10min后再灌组,脑电图有不同程度的恢复;结扎20和30min后再灌组,在观察的2h内,未见脑电图恢复。光镜检查:缺血5和10min后,未见明显形态学变化;缺血20和30min后,有轻到中度神经细胞变性;再灌后上述变化明显加重,并伴有显著水肿。
In this study, healthy and male Wistar rats were used to ligate left and right subclavian artery and common carotid artery under direct vision. The duration of ligation was 5, 10, 20 and 30 minutes, respectively. Dynamic changes of EEG, ECG and dizzy holes were observed during the experiment. After the experiment, blood vessels were ligated accurately, and the distribution of dye in the brain was observed by aspiration of ascending aorta. The brain was fixed by perfusion and fixation Morphological examination. The results showed that all animal blood vessels ligation site accurate injection dye confirmed that the use of the method of cerebral ischemia and reperfusion caused by reliable results. EEGs of all animals were completely inhibited within 5 seconds, 10 minutes, and 30 minutes of continuous ligation after a few seconds to more than ten seconds after vascular ligation. After 5 and 10 minutes of ligation, reperfusion group, brain There are different degrees of electrical restoration; ligation 20 and 30min after reperfusion group, observed within 2h, no EEG recovery. Light microscopy: 5 and 10min after ischemia, no obvious morphological changes; 20 and 30min after ischemia, mild to moderate nerve cell degeneration; After reperfusion, the above changes were significantly aggravated, accompanied by significant edema.