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目的:探讨ANXA2P1在神经胶质细胞瘤(GBM)组织中的表达及其在U87细胞中的生物学功能。方法:通过TCGA数据库信息分析GBM组织中ANXA2P1的表达。利用细胞转染法上调或下调GBM组织或U87细胞中ANXA2P1的表达，通过CCK-8实验检测U87细胞的增殖能力，Western blotting实验检测U87细胞信号通路蛋白表达的变化。结果:通过TCGA数据库分析发现，ANXA2P1在高级别的胶质瘤组织中表达水平明显高于低级别的胶质瘤组织(n P<0.01)。在胶质瘤组织中ANXA2的表达水平与ANXA2P1的表达水平呈正相关(n r＝0.752，n P<0.01)。过表达ANXA2P1的U87细胞增殖能力明显高于对照组，而Si-ANXA2P1 U87细胞的增殖能力明显低于对照组(n P<0.05或n P<0.01)。转染72 h后，过表达ANXA2P1细胞中p-S6K蛋白表达水平高于对照组(n P<0.05)，而Si-ANXA2P1细胞中p-S6K蛋白表达水平低于对照组(n P<0.05)。n 结论:ANXA2P1可能通过mTOR信号通路促进U87细胞的增殖。“,”Objective:To investigate the expression of annexin A2 pseudogene 1 (ANXA2P1) in glioblastoma (GBM) tissues and its biological function in U87 cells.Methods:The expression level of ANXA2P1 in GBM tissues was analyzed by Cancer Genome Atlas (TCGA). Cell transfection was used to up-regulate or down-regulate the expression of ANXA2P1 in GBM tissues or U87 cells. Cell Counting Kit-8 (CCK-8) was used to detect the proliferation ability of U87 cells. Western blotting was used to detect the changes of protein expression in signaling pathway of U87 cells.Results:The TCGA analysis showed that the expression level of ANXA2P1 in high-level GBM tissues was significantly higher than that in low-level GBM tissues (n P<0.01). In GBM tissues, the expression level of ANXA2 was positively correlated with that of ANXA2P1 (n r=0.752, n P<0.01). The proliferation capacity of U87 cells with ANXA2P1 overexpression was higher than that of the control group, while the proliferation capacity of U87 cells transfected by Si-ANXA2P1 was lower than that of the control group. At 72 h after transfection, the expression level of p-S6K protein in the ANXA2PI-overexpressed cells was higher than that in the control group (n P<0.05), while the expression level of p-S6K protein in the Si-ANXA2P1 cells was lower than that in the control group (n P<0.05).n Conclusion:ANXA2P1 may promote the proliferation of U87 cells through mTOR signaling pathway.