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在强光胁迫下,采用水培法,使用外源NO和去除内源NO措施,研究了NO对2个高羊茅(Arid3和Houndog5)品种的抗氧化系统的调节作用。结果表明,Arid3的内源NO比对照增加201.5%,而Houndog5仅增加21.1%。Houndog5发生严重氧化损伤,电解质渗出率和丙二醛(MDA)含量分别比对照增加72.6%和85.1%,而Arid3受到的伤害较轻,分别比对照增加36.1%和30.1%。使用0.2mmol.L-1的NO专一清除剂2,4-羧基苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物(PTIO)清除内源NO,加剧强光胁迫对Arid3与Houndog5氧化伤害。应用0.1mmol.L-1的外源NO供体硝普钠(SNP)能降低强光胁迫造成的Arid3和Houndog5叶片脂氧合酶(LOX)活性,降低超氧自由基(O2.-)产生速率和质膜相对透性的增加以及MDA和H2O2的累积;同时,0.1mmol.L-1的SNP还能够诱导过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸氧化物酶(APX)和谷胱甘肽还原酶(GR)活性。而外源NO的保护作用被PTIO逆转。上述结果暗示,NO可能作为一个活性分子诱导抗氧化物酶的活性,减轻强光胁迫下2个高羊茅品种氧化损伤。
Under the light stress, the regulation of NO on the antioxidant system of two tall fescue (Arid3 and Houndog5) cultivars was studied by hydroponics, using exogenous NO and removing endogenous NO. The results showed that endogenous NO of Arid3 increased by 201.5% compared with the control, while that of Houndog5 increased by only 21.1%. Houndog5 serious oxidative damage, electrolyte leakage and malondialdehyde (MDA) content increased by 72.6% and 85.1% compared with the control, the less the damage Arid3, increased 36.1% and 30.1% compared with the control. Using 0.2mmol.L-1 NO specific scavenger 2,4-carboxybenzene-4,4,5,5-tetramethylimidazole-1-oxo-3-oxide (PTIO) High light stress on Arid3 and Houndog5 oxidative damage. Application of 0.1mmol.L-1 exogenous nitric oxide donor sodium nitroprusside (SNP) could reduce the LOX activity of Arid3 and Houndog5 leaves and reduce the production of superoxide radical (O2.-) (POD), superoxide dismutase (SOD), catalase (CAT), catalase (CAT) and catalase (CAT), ascorbate oxidase (APX) and glutathione reductase (GR) activity. While the protective effect of exogenous NO was reversed by PTIO. The above results suggest that NO may act as an active molecule to induce the activity of antioxidant enzymes and alleviate the oxidative damage of two tall fescue cultivars under light stress.