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１９８９～１９９３年从２２８例急性驰缓性麻痹病例（ＡＦＰ）便、１５９６分正常人群便及８５份城市生活污水中分离出１１０株Ｐｏｌｉｏ病毒，９７株其它病毒。对１１０株Ｐｏｌｉｏ病毒全部用抗Ｐｏｌｉｏ病毒１、２、３型３组单克隆抗体（Ｍｃａｂ）进行中和分析，对其中３０株病毒同时用ＰＣＲ方法对其ＲＮＡ进行了分析。结果１１０株Ｐｏｌｉｏ病毒只有１株９０—３５＋３Ｓ病毒为Ｐ１型野毒株，其它１０９株全为Ｓａｂｉｎ疫苗株。１９９１～１９９３年以来连续３年未发现Ｐｏｌｉｏ野病毒。此结果为辽宁省１９９３年７月消灭由Ｐｏｌｉｏ野病毒引起的麻痹病提供了可靠的病原学依据。
From 1989 to 1993, 110 Polio viruses and 97 other viruses were isolated from 228 patients with acute flaccid paralysis (AFP), 1596 normal subjects and 85 municipal sewage. All of the 110 Polio viruses were neutralized with anti-Polio virus type 1, 2, 3 and 3 monoclonal antibodies (Mcab), and 30 of them were simultaneously analyzed by PCR for their RNA. Results Only one strain of 90-35 + 3S was found to be type P1 wild-type and the other 109 strains were all Sabin-type strains. Polio wild virus was not found for three consecutive years from 1991 to 1993. This result provided a reliable etiological basis for Liaoning Province in July 1993 to eliminate paralytic diseases caused by Polio wild virus.