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目的:研究寒冷所诱导的机体损伤作用,明确寒冷引发机体损伤的部分机制以及预防性药物对机体处于寒冷环境中的保护作用。方法:通过低温试验、ATP检测、电镜观察等确定寒冷对机体造成的损伤;通过总抗氧化能力、CAT含量、谷胱甘肽过氧化物酶含量、SOD水平、MDA水平等的检测确定氧化应激在寒冷暴露对机体损伤中的作用及机制;并确证复方中药制剂在寒冷损伤氧化应激状态下对机体的保护作用。结果:1.急性-15±1℃寒冷环境暴露可引起机体内肝脏组织能量代谢障碍,ATP生成减少,肝细胞线粒体损伤,溶酶体增多等机体损伤的现象;2.寒冷应激可导致机体的总抗氧化能力、CAT显著减少,而氧化应激终产物之一的丙二醛(malondialdehyde,MDA)显著增多(说明肝脏组织出现明显的氧化应激过程),并且出现抗氧化能力下降。3.预先给予药物干预后,机体抗氧化能力显著增加。结论:1.寒冷可以诱导机体肝脏明显损伤。2.氧化应激是寒冷诱导机体损伤的关键机制之一。3.复方中药制剂可以增加机体在寒冷应激条件下的抗氧化能力,从而产生保护作用。
OBJECTIVE: To study the role of cold induced injury in vivo, clarify some mechanisms of cold-induced injury and the protective effect of preventive drugs on the cold environment. Methods: The cold damage to the body was determined by low temperature test, ATP test and electron microscope observation. The oxidative stress was determined by total antioxidant capacity, CAT content, glutathione peroxidase content, SOD level and MDA level Stimulate the role of cold exposure on the body injury and mechanism; and confirm the compound Chinese medicine preparations in cold damage oxidative stress on the body’s protective effect. Acute -15 ± 1 ℃ cold environment can cause liver tissue energy metabolism disorder, ATP production decreased, mitochondrial damage in liver cells, increased lysosome and other body damage phenomenon; 2. Cold stress can lead to body (P <0.05). CAT decreased significantly, while malondialdehyde (MDA), one of the end products of oxidative stress, increased significantly (indicating obvious oxidative stress in liver tissue), and the antioxidant capacity decreased. 3. Pre-given drug intervention, the body’s antioxidant capacity increased significantly. Conclusion: 1. Cold can induce significant liver injury. Oxidative stress is one of the key mechanisms of cold-induced body damage. 3. Compound traditional Chinese medicine can increase the body’s antioxidant capacity in cold stress conditions, resulting in a protective effect.