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通过２个不同品系小鼠间进行皮肤移植，研究免疫系统产生特异耐受的机制。要获得对异体抗原的特异性耐受，必须包括对外周淋巴器官中免疫效应细胞的特异杀伤，并同时诱导中枢免疫器官将其作为“自我”物质进行识别，导致系列克隆流产。以ＢＡＬＢ／Ｃ鼠为受体，以Ｃ５７ＢＬ／６（简称Ｃ５７）鼠为供体，用包裹蓖麻毒素蛋白（ｒｉｃｉｎ）的供体淋巴细胞作为“导弹毒剂”，对受体产生的排异供体移植抗原的Ｔ，Ｂ淋巴细胞进行定向杀伤，并事先用供体淋巴细胞对受体进行胸腺内注射，以使得识别移植抗原的Ｔ淋巴细胞的克隆流产。实验组移植皮片平均存活超过６０ｄ（天）（对照组为５ｄ），ｔ检验证明差异极其显著。单向混合淋巴细胞培养的ＭＴＴ比色分析结果为导向毒细胞的定向杀伤功能和供体淋巴细胞胸腺注射的Ｔ细胞克隆衰减功能提供了细胞学证据。
Through two different strains of mice between the skin graft, immune system to study the mechanism of specific tolerance. To achieve specific tolerance to alloantigens, it is necessary to include specific killing of immune effector cells in peripheral lymphoid organs and to induce central immune organs to recognize them as “self” substances, resulting in a series of clonal abortions. To BALB / C mice as recipients, C57BL / 6 (referred to as C57) mice as donors, donor rhabdomyosin protein (ricin) donor lymphocytes as a “missile poison” The T, B lymphocytes of the somatically transplanted antigen are targeted and killed, and the recipient is previously thymus injected with the donor lymphocyte so that clonal abortion of the T lymphocyte that recognizes the transplanted antigen occurs. In the experimental group, the average graft graft survival more than 60 days (control group for 5 days), t test showed that the difference was extremely significant. MTT colorimetric analysis of one-way mixed lymphocyte cultures provides cytological evidence for the direct killing of cytotoxic cells and the attenuation of T cell clones by thymic injection of donor lymphocytes.