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为了探讨一氧化氮在微波预处理提高小麦抗镉胁迫中的作用,利用微波(126mW.cm-2)辐照小麦种子10 s,待其长至一叶一心时,用150μmol·L-1CdCl2胁迫其幼苗,并通过添加一氧化氮专一清除剂cPTIO(0.5%,w/v),研究一氧化氮对微波预处理小麦幼苗脂质过氧化、抗氧化酶活性、光合色素含量及幼苗生长的影响。结果表明:微波预处理可使镉胁迫的小麦幼苗丙二醛含量显著降低(P<0.05),超氧化物歧化酶、过氧化物酶、过氧化氢酶活性、一氧化氮、叶绿素a、叶绿素b、类胡萝卜素含量和株高、根长、地上部干重、根干重显著增加(P<0.05);经过适当剂量微波辐照镉胁迫小麦幼苗再加外源cPTIO处理能有效逆转微波对镉胁迫小麦幼苗的防护作用。说明微波对镉胁迫损伤小麦幼苗起到一定的防护作用主要是通过NO起作用。
In order to investigate the effect of nitric oxide pretreatment to improve the resistance of wheat to cadmium stress, the wheat seeds were irradiated by microwave (126 mW · cm-2) for 10 s and treated with 150 μmol·L-1CdCl2 The seedlings were treated with cPTIO (0.5%, w / v), a specific nitric oxide scavenger to study the effects of nitric oxide on lipid peroxidation, antioxidant enzyme activity, photosynthetic pigment content and seedling growth of wheat seedlings under microwave pretreatment influences. The results showed that microwave pretreatment could significantly reduce the malondialdehyde content of wheat seedlings exposed to cadmium stress (P <0.05), and the activities of superoxide dismutase, peroxidase, catalase, nitric oxide, chlorophyll a, chlorophyll b, the carotenoid content and plant height, root length, aboveground dry weight and root dry weight increased significantly (P <0.05). After cadmium stress, the wheat seedlings treated with cadmium stress and exogenous cPTIO treatment could effectively reverse the effects of microwave Protective effects of cadmium stress on wheat seedlings. This indicated that microwave could play a protective role against cadmium stress-damaged wheat seedlings mainly through NO.