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目的研究膳食硒对大鼠肝脏中５′－脱碘酶（５′－ＤＩ）的活性影响。方法实验组饲料附加硒０．５ｐｐｍ。对照组不附加硒。１２周后测血硒、血浆谷胱甘肽过氧化物酶（ＧＰＸ）以及肝５′－脱碘酶活性。后者测定用Ｃｈｏｐｒａ氏放射免疫法。结果加硒组大鼠肝脏５′－脱碘酶活性平均为４２．１８±１７．４２ＰｍｏｌＴ３／ｍｉｎ，ｍｇＰｒｏｔｅｉｎ，明显高于对照组均值１３．２４±４．４８ＰｍｏｌＴ３／ｍｉｎ，ｍｇＰｒｏｔｅｉｎ，Ｐ＜０．００１。同时平均全血硒含量明显高于对照组２．４７倍。血浆ＧＰＸ明显高于对照组，Ｐ＜０．０５。结论５′－脱碘酶活性与谷胱甘肽过氧化物酶均是含硒酶，在生理上起着重要作用。
Objective To study the effect of dietary selenium on 5’-deiodinase (5’-DI) activity in rat liver. Methods Experimental group feed additional selenium 0.5ppm. The control group did not attach selenium. Plasma selenium, plasma glutathione peroxidase (GPX) and hepatic 5’-deiodinase activity were measured after 12 weeks. The latter test using Chopra’s radioimmunoassay. Results The liver 5’-deiodinase activity in the selenium-added group was 42.18 ± 17.42PmolT3 / min, mgProtein, which was significantly higher than that in the control group (13.24 ± 4.48PmolT3 / min, mgProtein, P <0). 001. At the same time the average whole blood selenium content was significantly higher than the control group 2.47 times. Plasma GPX was significantly higher than the control group, P <0.05. Conclusion Both 5’-deiodinase activity and glutathione peroxidase are selenium-containing enzymes and play an important physiological role.