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目的了解2012年新疆乌鲁木齐市婴幼儿轮状病毒流行特征及基因型别特点。方法收集2012年1—12月在乌鲁木齐市第一人民医院就诊住院的0～60月龄婴幼儿腹泻患儿粪便标本300份,用酶联免疫法进行轮状病毒抗原检测,检测结果阳性标本用逆转录-聚合酶链式反应法进行G/P基因分型鉴定。结果在300份粪便标本中,检测出阳性样本131份,阳性率为43.7%;在G基因分型鉴定中,G9型为优势株,占53.44%,其次为G2型,占17.56%,G3和G1型分别占12.98%和9.92%,G型混合感染占6.11%;在P基因型鉴定中,P[8]型占绝对优势,占73.28%,其次为P[4]型,占20.61%,混合感染占5.34%;G9P[8]型是最常见的G/P组合,占48.85%,其次为G2P[4]型,占17.56%。结论 2012年乌鲁木齐轮状病毒流行优势毒株发生了从G3到G9的漂移,G9P[8]成为该地区最主要的基因型。
Objective To understand the prevalence and genotype characteristics of infants and young children in Urumqi in 2012. Methods A total of 300 stool samples from 0 to 60 months old infantile diarrhea children hospitalized in Urumqi First People’s Hospital from January to December in 2012 were collected and tested for rotavirus antigen by enzyme-linked immunosorbent assay Reverse transcription - polymerase chain reaction for G / P genotyping. Results Among 300 samples of stool samples, 131 samples were positive, with a positive rate of 43.7%. Among G genotypes, G9 genotype was predominant (53.44%), followed by G2 (17.56%), G3 and G1 type accounted for 12.98% and 9.92% respectively, and G type mixed infection accounted for 6.11%. In the P genotype identification, P [8] type accounted for 73.28%, followed by P [4] type, accounting for 20.61% Mixed infection accounted for 5.34%; G9P [8] type is the most common G / P combination, accounting for 48.85%, followed by G2P [4] type, accounting for 17.56%. Conclusions The epidemic strain of rotavirus from Urumqi occurred from G3 to G9 in 2012, and G9P [8] became the most important genotype in this area.