论文部分内容阅读
根据沙眼衣原体内源性质粒和鹦鹉热衣原体16S rRNA基因的序列,合成了与之互补的两对引物,分别扩增517 bp和208 bp的DNA片断。经沙眼衣原体标准株TE 55和鹦鹉热衣原体GPIC验证,引物与原设计相符。实验表明,两种引物均能扩增各种血清型沙眼包涵休性结膜炎衣原体,rRNA引物还能扩增鹦鹉热衣原体。经对17例临床诊断为沙眼的标本进行检测,并与ELISA和免疫荧光法对同一标本的检测的结果进行比较,表明多聚酶链反应技术可用于沙眼衣原体眼部感染的快速检测。
According to the sequences of 16S rRNA gene of Chlamydia trachomatis endogenous plasmids and Chlamydia psittaci, two complementary primers were synthesized, and the 517 bp and 208 bp DNA fragments were amplified respectively. Chlamydia trachomatis standard strain TE 55 and Chlamydia psittaci GPIC validation, primers consistent with the original design. Experiments show that both primers can amplify all serotype trachoma inclusive of conjunctivitis conjunctivitis chlamydia, rRNA primer can also amplify psittaci hot chlamydia. Seventeen clinically diagnosed as trachoma specimens were tested and the results of ELISA and immunofluorescence assay on the same specimen were compared, indicating that polymerase chain reaction can be used for the rapid detection of ocular trachoma in Chlamydia trachomatis.