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目的 通过对临产前孕鼠行不同程度的缺氧,找出产前缺氧性适应最合适的缺氧浓度,从而建立产前缺氧性适应动物模型。方法 将孕22天的临产前孕鼠随机分为7组,每只孕鼠两次放入自制的缺氧性适应密封仓中,氧浓度分别降至18％（Ⅱ组）、17％（Ⅲ组）、16％（Ⅳ组）、15％（Ⅴ组）、14％（Ⅵ组）、13％（Ⅶ组）,两次乏氧间隔5分钟,对照组（Ⅰ组）氧浓度21％。Ⅰ-Ⅶ组孕鼠的缺氧适应时间分别为:第一次10min、5min、7.5min、9.83min、11.5min、13.17min、14min;第二次10min、9.33min、11min、15.17min、13.33min、17min、18min。比较各组娩出的新生鼠耐缺氧时间,并应用尼氏染色和透射电镜对各组新生鼠脑组织进行观察,找出最合适的缺氧浓度。结果 Ⅰ-Ⅴ组娩出的均为正常新生鼠;Ⅵ组娩出的55只新生大鼠,皮肤颜色青紫、运动减少10只（18％）,部分新生鼠脑组织正常神经细胞数量减少,部分细胞肿胀,线粒体扩张,可见凋亡细胞;Ⅶ组娩出的52只新生大鼠中,死亡11只（21％）,14只皮肤青紫、肢体运动减少（27％）,部分新生鼠脑组织病理学检查异常。Ⅳ、Ⅴ、Ⅵ组新生鼠耐乏氧时间较对照组明显延长,Ⅳ组、Ⅴ组两组间新生鼠耐乏氧时间有统计学差异。结论2次使孕鼠缺氧（无CO2蓄积）,氧浓度15％,中间吸入新鲜空气5分钟是产前缺氧
OBJECTIVE: To find out the most suitable hypoxic concentration for prenatal hypoxia adaptation through prenatal pregnant rats with varying degrees of hypoxia to establish prenatal hypoxic adaptation animal model. Methods Pregnant prenatal pregnant rats of 22 days old were randomly divided into 7 groups. Each pregnant rat was placed in a self-made anoxic chamber, the concentration of oxygen decreased to 18% (group Ⅱ), 17% (Ⅲ (Group Ⅳ), 15% (group V), 14% (group Ⅵ) and 13% (group Ⅶ). The oxygen exposure was 21% in the control group (group Ⅰ). The hypoxia adaptation time of pregnant rats in group Ⅰ-Ⅶ were 10min, 5min, 7.5min, 9.83min, 11.5min, 13.17min, 14min respectively; the second 10min, 9.33min, 11min, 15.17min, 13.33min , 17min, 18min. The hypoxia time of newborn rats was compared between the two groups. The brain tissues of neonatal rats in each group were observed by Nissl staining and transmission electron microscope to find out the most appropriate hypoxia concentration. Results All the newborn rats were delivered from group Ⅰ-Ⅴ. Fifty-five newborn rats in group Ⅵ had purple-green skin color and decreased exercise by 10 (18%). The number of normal nerve cells in some neonatal rats decreased and some of them were swollen , Mitochondria dilated and apoptotic cells were observed. Among the 52 neonatal rats in group Ⅶ, 11 died (21%), 14 were bruising and the limb movement was decreased (27%). Some of the neonatal rats had abnormal histopathological examination . The hypoxia-tolerance time of neonates in Ⅳ, Ⅴ and Ⅵ groups were significantly longer than that in control group, and there was significant difference in the hypoxia tolerance time of neonatal rats in groups Ⅳ and Ⅴ. Conclusions The pregnant rats were hypoxic (without CO2 accumulation) 2 times, oxygen concentration 15%, fresh air inhalation 5 minutes in the middle was prenatal hypoxia