论文部分内容阅读
为筛选防治牡丹红斑病的杀菌剂,采用涂布平板法测定了多菌灵、戊唑醇、嘧菌酯对病菌分生孢子形成和萌发的影响。结果表明:3种杀菌剂对病菌孢子萌发均有很强的抑制作用,浓度越高,抑制作用越强,以嘧菌酯最强,1μg/mL浓度处理其孢子萌发率低于10%,5μg/mL处理孢子完全不能萌发;3种杀菌剂均可显著抑制病菌芽管的伸长生长,1μg/mL处理下孢子萌发24 h后芽管长度均不足10μm,5μg/mL处理对芽管伸长的抑制率达到90%;3种杀菌剂可使分生孢子及芽管发生畸形,多菌灵的致畸作用最强,0.1μg/mL处理即表现出明显的致畸作用,而戊唑醇致畸作用较弱;嘧菌酯和戊唑醇对病菌产孢结构形成和分生孢子产生数量有强烈的抑制作用,0.05μg/mL浓度下即完全不产孢,但多菌灵在0.05μg/mL浓度下则促进病菌产孢结构形成及产孢量增加。表明3种杀菌剂均可作为保护剂在病害发生前期喷施,但在流行期应尽量使用嘧菌酯和戊唑醇,以减少病菌再侵染数量。
In order to screen the fungicide for preventing and curing peony erythematosus, the effects of carbendazim, tebuconazole and azoxystrobin on the conidial formation and germination of the bacteria were determined by the coating plate method. The results showed that the three fungicides had a strong inhibitory effect on spore germination. The higher the concentration was, the stronger the inhibition was. The highest spore germination rate was less than 10% at the concentration of 1μg / mL, / mL treatment of spores can not germination; 3 kinds of fungicides can significantly inhibit the growth of germ tube germination growth, spores germination at 1μg / mL 24 hours germ tube length less than 10μm, 5μg / mL treatment of germ tube elongation Of the fungicides can conidia and germ tube deformity, the most teratogenic effect of carbendazim, 0.1μg / mL treatment showed significant teratogenic effects, and tebuconazole Teratogenicity was weaker. Azoxystrobin and tebuconazole had a strong inhibitory effect on sporulation and sporulation of spores. At 0.05μg / mL, sporulation did not produce spores at all, but carbendazim was inhibited at 0.05μg / mL concentration is to promote the formation of bacteria sporulation structure and spore production increased. All the three kinds of fungicides can be used as protective agent in the pre-disease stage, but azoxystrobin and tebuconazole should be used as far as possible during the epidemic period to reduce the number of re-infection.