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单以乙肝病毒S区基因疫苗 (pCR3 1 S)或联合IL 2真核表达载体 (pDOR IL 2 )注射BALB/c小鼠 (H 2 d)股四头肌 ,ELISA法检测小鼠血清抗HBs,4h51Cr释放法检测小鼠脾细胞CTL活性。免疫 8周后 ,单注射pCR3 1 S及共注射IL 2真核表达载体的小鼠血清 45 0nmA值分别为 1 2 4± 0 1及 1 98± 0 17。CTL细胞杀伤活性分别为 (5 0 5± 6 4) %、 (6 1 9± 7 1) % ,两组均有明显差异 (P <0 0 1)。脾细胞悬液经抗CD4+ 单克隆抗体处理后CTL细胞杀伤活性分别为 (4 8 3± 5 9) %、 (5 6 2±6 1) % ,抗CD8+ 单克隆抗体处理后分别为 (10 6± 1 4) %、 (13 6± 1 3) %。结果表明 ,IL 2的真核表达载体能够提高小鼠对DNA疫苗的免疫应答 ,CTL细胞杀伤活性主要由CD8+ 执行。基因疫苗可能用于预防及治疗HBV感染。
BALB / c mice (H 2 d) quadriceps were injected with hepatitis B virus S-region gene vaccine (pCR3 1 S) or IL-2 eukaryotic expression vector (pDOR IL 2) , 4h51Cr release assay CTL activity of mouse spleen cells. After 8 weeks of immunization, the 45 0 nmA values of single injection of pCR3 1 S and co-injection of IL2 eukaryotic expression vector were 120 4 ± 0 1 and 1 98 ± 0 17, respectively. The cytotoxicity of CTL was (5 0 5 ± 6 4)% and (6 1 9 ± 7 1)%, respectively, with significant difference between the two groups (P 0 01). The cytotoxicity of CTL cells in splenocyte suspension treated with anti-CD4 + monoclonal antibody were (483 ± 59)% and (562 ± 6 1)%, respectively, and the anti-CD8 + ± 1 4)%, (13 6 ± 1 3)%. The results showed that the eukaryotic expression vector of IL 2 can improve the immune response of mice to DNA vaccine. The CTL cell killing activity is mainly performed by CD8 +. Gene vaccines may be used to prevent and treat HBV infection.