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结合壳聚糖(CS)和聚己内酯(PCL)二者的优点,以静电纺丝的方法制备了CS/PCL血管支架。采用SEM和电子万能试验机检测了该支架的结构和力学性能,将内皮祖细胞(EPCs)与该支架膜复合培养,评估了该血管支架维持细胞黏附、繁殖和分化的能力。SEM结果显示:通过静电纺丝可以得到多孔、类似于天然细胞外基质的直径约400 nm的纤维微结构;当CS与PCL质量比为0.5时,静电纺丝所制备的CS/PCL血管支架弹性最大形变达到31.64%,应力-应变曲线显示其弹性变形能力较强;EPCs在CS/PCL血管支架黏附率可达95.1%,荧光显微镜观察结果也显示了CS/PCL血管支架利于细胞黏附、生长。
Combined with the advantages of both chitosan (CS) and polycaprolactone (PCL), CS / PCL vascular stents were prepared by electrospinning. The structure and mechanical properties of the scaffolds were examined by SEM and electronic universal testing machine. The endothelial progenitor cells (EPCs) were co-cultured with the scaffolds and the ability of the scaffolds to adhere, multiply and differentiate was evaluated. The results of SEM showed that porous nanofibers with diameter about 400 nm were obtained by electrospinning. When the mass ratio of CS to PCL was 0.5, the elasticity of CS / PCL stent prepared by electrospinning The maximal deformation reached 31.64%, and the stress-strain curve showed a strong elastic deformation. The adhesion rate of EPCs in CS / PCL stent was 95.1%. The results of fluorescence microscope also showed that CS / PCL stent could promote cell adhesion and growth.