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了解江苏省南京市和无锡市两地夏秋季急性胃肠炎人星状病毒(HastV)的基因亚型。收集2015~2016年6~11月江苏省南京市和无锡市两地夏秋季急性胃肠炎患者粪便标本,采用real-time RT-PCR法检测粪便中HAstV的病毒核酸,对阳性标本ORF2区进行RT-PCR扩增及测序,与GenBank公开的序列进行比对,分析基因亚型情况。江苏省南京市和无锡市两地夏秋季急性胃肠炎HAstV感染的比例为15.26%,其中50%为合并其它急性胃肠炎病毒感染,HAstV感染以秋季流行为主,包含HAstV-1a(group 2)亚型和HAstV-4c基因亚型,并且这2个亚型在两地均有分布。HastV在婴幼儿人群中感染率较高;HAstV-1a亚型为在江苏省南京市和无锡市两地首次发现及报道的亚型,可能为欧美输入性毒株,本研究为进一步了解国内HAstV分子流行特征提供依据。
To understand the genotypes of summer and autumn acute gastroenteritis human astroviruses (HastV) in Nanjing and Wuxi, Jiangsu Province. Collect stool specimens from patients with acute gastroenteritis in summer and autumn in Nanjing and Wuxi, Jiangsu Province from June to November in 2015 ~ 2016 and detect the HAstV viral nucleic acid in the stool by real-time RT-PCR, RT-PCR amplification and sequencing, and GenBank published sequence comparison, analysis of gene subtypes. The proportion of HAstV infection in summer and autumn in Nanjing and Wuxi in Jiangsu Province was 15.26%, 50% of them were complicated by other acute gastroenteritis virus infection. The infection of HAstV mainly occurred in autumn, including HAstV-1a (group 2) subtypes and HAstV-4c gene subtypes, and these two subtypes are distributed in both places. HastV infection in infants and young children is higher; HAstV-1a subtypes are the first subtypes found and reported in Nanjing and Wuxi in Jiangsu Province, which may be imported from Europe and the United States. In order to further understand the domestic HAstV Molecular epidemic characteristics provide the basis.