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以MS培养基为基本培养基,以紫苏的幼嫩茎尖、带叶腋芽和嫩叶为外植体,比较了不同激素及其组合对紫苏愈伤组织诱导的影响。结果表明,最佳培养基组合为:诱导培养基MS+6-BA3.0mg/L+NAA0.5mg/L;再分化培养基MS+6-BA3.0mg/L;增殖培养基MS+6-BA2.0mg/L;生根培养基1/2MS+NAA0.2mg/L。
Taking MS medium as the basic medium, young shoot tips of Perilla frutescens, axillary buds with leaves and young leaves were used as explants to compare the effects of different hormones and their combinations on the callus induction of Perilla frutescens. The results showed that the best combination of medium was: MS + 6-BA3.0mg / L + NAA0.5mg / L; MS + 6-BA3.0mg / BA2.0mg / L; rooting medium 1 / 2MS + NAA0.2mg / L.