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目的:建立同时测定复方牛磺酸滴眼液中牛磺酸和氨基己酸含量的高效液相色谱-示差折光检测器法.方法:采用Waters氨基键合硅胶柱(250 mm×4.6 mm,5 μm),流动相为水-乙腈(40:60),流速为0.8 ml·min-1,柱温为35.0℃,检测器参比池和检测池温度均为35.0℃,进样量为20 μl.结果:牛磺酸和氨基己酸质量浓度在0.05 ~2.00 mg·ml-1(r均为0.9999)范围内与峰面积呈良好的线性关系,平均回收率分别为98.57%(RSD = 1.07%,n = 9) 和100.69%(RSD = 0.75%,n = 9).结论:该方法准确、简便、快速,适用于复方牛磺酸滴眼液的质量控制.“,”Objective:To establish an HPLC-RID method for the simultaneous determination of taurine and aminocaproic acid in compound taurine eye drops. Methods:A Waters NH2column(250 mm ×4.6 mm,5 μm) was used, the mobile phase was water-acetonitrile (40:60) at a flow rate of 0.8 ml·min-1, the column temperature was 35.0℃, and the temperatures of the detector reference cell and the sample cell were both set at 35.0℃,The injection volume was 20 μl. Results:The linear ranges of taurine and aminocaproic acid were 0.05-2.00 mg·ml-1(r = 0.999 9). The average recovery was 98.57% (RSD = 1.07%,n= 9) and 100.69% (RSD =0.75%,n =9),respectively. Conclusion:The established method is accurate,simple and rapid, and suitable for the quality control of compound taurine eye drops.