论文部分内容阅读
以‘吉塞拉5号’樱桃组培苗为试材,研究短期低氧胁迫下氮对樱桃幼苗根系抗氧化酶活性及线粒体功能的影响。通过溶氧调节仪人为控制营养液溶氧浓度为2mg·L-1,NO3-浓度共设4个水平:0、7.5、15.0、22.5mmol·L-1,以1/2 Hoagland营养液正常低氧处理(NO3-浓度7.5mmol·L-1)为对照。结果表明:各处理SOD、POD、CAT酶活性均呈现先升高后降低的趋势。与对照相比,营养液加氮处理的SOD、POD、CAT酶活性升高,膜质过氧化程度减轻,且氮浓度越大酶活性升高幅度越大,而营养液缺氮处理的酶活性降低,膜质过氧化程度加重;营养液缺氮处理樱桃根系线粒体中的H2O2和MDA含量、O2·-生成速率升高,而营养液加氮处理可降低O2.-的产生及MDA含量,提高线粒体的氧化磷酸化水平,保护樱桃根系线粒体膜的完整性,对线粒体呼吸功能的破坏明显减轻,且NO3-离子浓度高时效果更好,说明适当提高营养液中的氮素水平可以缓解低氧胁迫对樱桃根系的伤害。
The effects of nitrogen on antioxidant enzyme activity and mitochondrial function in roots of cherry seedlings under short-term hypoxia stress were studied using the tissue culture seedlings of ’Gisela 5’ cherry. Dissolved oxygen regulator artificial control nutrient solution dissolved oxygen concentration of 2mg · L-1, NO3- concentration of a total of four levels: 0,7.5,15.0,22.5mmol·L-1 to 1/2 Hoagland nutrient solution normal low Oxygen treatment (NO3- concentration of 7.5mmol · L-1) as a control. The results showed that the enzyme activities of SOD, POD and CAT increased first and then decreased. Compared with the control, the activities of SOD, POD and CAT increased with the addition of nitrogen and the degree of membrane peroxidation decreased. The higher the nitrogen concentration, the greater the increase of enzyme activity, while the activity of nitrogen deficiency Decreased, and the degree of membrane hyperperoxidation aggravated. The content of H2O2 and MDA in the mitochondria of cherry root in nutrition-deficient treatment solution increased and the O2 · - generation rate increased. However, the addition of nitrogen in the nutrient solution reduced the production of O2.- and the content of MDA Mitochondrial oxidative phosphorylation level, to protect the integrity of the mitochondrial membrane of cherry root mitochondrial respiratory function damage significantly reduced, and NO3- ion concentration is better, indicating that the appropriate increase in nutrient solution of nitrogen levels can alleviate hypoxia Stress on the cherry root damage.