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目的探讨胃癌及其癌前病变中端粒酶的表达。方法对纤维内窥镜下活检的胃组织采用对TRAP方法加以改进的银染TRAP法，检测了端粒酶活性。结果29例胃癌中阳性率（23／29）82．7％：胃溃疡8例及胃息肉1例皆未检测到端粒酶活性，慢性萎缩性胃炎伴肠上度化生（AGIM）8例中4例有端粒酶表达。胃癌及AGIM端粒酶阳性率与良性病变阳性率比较差异显著（P＜0．05）。结论银染TRAP法具有检测标本用量少，周期短，灵敏度高，特异性强等优点。对胃活检组织进行端粒酶检测在胃癌诊断中具有重要意义。对端粒酶检测阳性者应加强随访。
Objective To investigate the expression of telomerase in gastric cancer and its precancerous lesions. Methods The telomerase activity was detected in the gastric tissue biopsy under fiberoptic endoscopy using the silver staining TRAP method modified by TRAP. Results The positive rate (23/29) was 82.7% in 29 cases of gastric cancer: telomerase activity was not detected in 8 cases of gastric ulcer and 1 case of gastric polyp, and 8 cases of chronic atrophic gastritis with upper intestinal metaplasia (AGIM). In 4 cases, there was telomerase expression. The positive rates of telomerase in gastric cancer and AGIM were significantly different from those in benign lesions (P<0.05). Conclusion The silver staining TRAP method has the advantages of small amount of detection, short cycle, high sensitivity and strong specificity. The detection of telomerase in gastric biopsies is of great significance in the diagnosis of gastric cancer. Positive telomerase test should be followed up.