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背景:海马组织中乙酰胆碱的代谢情况可在一定程度上反映与学习记忆相关的胆碱能系统的功能状况,并影响智能程度。目的:观察大鼠脑缺血再灌注后海马组织胆碱乙酰基转移酶活力的动态变化。设计:以实验动物为观察对象的随机对照实验研究。单位:承德医学院科研处。材料:实验于2002年在承德医学院中心实验室完成。W istar大鼠24只,体质量260～280g,雌雄各半,清洁级。方法:24只大鼠随机分为3组:①模型组。高脂血症大鼠,阻断大鼠双侧颈总动脉进行脑缺血再灌注。②假手术组。高脂血症大鼠仅做双侧颈总动脉分离术,不进行缺血再灌注。③正常对照组。不加任何处理。各组大鼠分别于术后第1,7,15d 断头取脑,用比色法测海马组织胆碱乙酰基转移酶活力。主要观察指标:大鼠脑海马组织胆碱乙酰基转移酶活力的测定。结果:24只大鼠进入结果分析,无脱失。①模型组大鼠第1天、第7天胆碱乙酰基转移酶活力较正常对照组明显降低犤模型组:(0.037±0.006)μm ol/g·s,(0.017±0.006)μm ol/g·s;正常组对照组:(0.054±0.003) μm ol/g·s,(0.058±0.006)μm ol/g·s,P <0.01犦。②模型组大鼠第7天胆碱乙酰基转移酶活力明显低于第1天,随着缺血再灌注损伤的修复,第15天胆碱乙酰基转移酶活力有所回升(0.039±0.007)μnm ol/g·s。③假手术组胆碱乙酰基转移酶活力与正常组无明显差异(P >0.05)。结论:单纯手术不会导致脑组织胆碱乙酰基转移酶的变化,脑缺血再灌注后可使海马胆碱乙酰基转移酶活力降低,使中枢胆碱能系统功能紊乱,可能是导致大鼠智能障碍的原因。
BACKGROUND: The metabolism of acetylcholine in the hippocampus may reflect the functional status of cholinergic system related to learning and memory to a certain extent and affect the intelligence. Objective: To observe the dynamic changes of choline acetyltransferase activity in rat hippocampus after cerebral ischemia-reperfusion. Design: Randomized controlled trial of experimental animals. Unit: Chengde Medical College Research Department. Materials: The experiment was completed in the laboratory of Chengde Medical College in 2002. W istar rats 24, body weight 260 ~ 280g, male and female, clean level. Methods: Twenty-four rats were randomly divided into three groups: ① model group. Hyperlipidemic rats were occluded from bilateral common carotid arteries for cerebral ischemia-reperfusion. ② sham operation group. Hyperlipidemic rats only bilateral carotid artery isolation, not for ischemia-reperfusion. ③ normal control group. Without any treatment. The rats in each group were sacrificed on the 1st, 7th and 15th day after operation respectively, and the activity of choline acetyltransferase in hippocampus was measured by colorimetry. MAIN OUTCOME MEASURES: Determination of choline acetyltransferase activity in rat hippocampus. Results: 24 rats entered the result analysis without loss. ① The choline acetyltransferase activity in model group was significantly lower than that in control group on day 1 and day 7 in model group (0.037 ± 0.006) μmol / g · s, (0.017 ± 0.006) μmol / g · S; normal control group: (0.054 ± 0.003) μmol / g · s, (0.058 ± 0.006) μmol / g · s, P <0.01 犦. ② The choline acetyltransferase activity in model group was significantly lower than that on the first day after the 7th day. With the repair of ischemia-reperfusion injury, the activity of choline acetyltransferase increased on the 15th day (0.039 ± 0.007) μ nm ol / g · s. ③ sham operation group choline acetyltransferase activity and normal group no significant difference (P> 0.05). CONCLUSIONS: Simple surgery does not lead to changes of choline acetyltransferase in brain tissue. After cerebral ischemia-reperfusion, the activity of choline acetyltransferase in the hippocampus is reduced and the central cholinergic system is dysfunctional. Causes of mental retardation.