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根据用豚鼠肾(GPK)匀浆吸收人血清中异嗜性抗体的结果,可将人异嗜性抗体分成GPK阳性和GPK阴性两类。在许多传染病和一些不明病因的疾病,如Chediak—Higashi综合症(C—H综合症),Kawasaki病和一些恶性肿瘤病人中,都存在着GPK阳性的异嗜性抗体。为了直接证明GPK匀浆与这些抗体的相互作用,本文作者采用混合凝集(MA)试验,即取100微升GPK悬液包被塑料板孔,然后加入100微升血清稀释液,20℃保温2小时后用磷酸缓冲液洗三次,再于每孔中加入100微升经胰酶消化的0.1％(v/v)羊或牛红细胞,20℃保
Human heterophile antibodies can be divided into GPK positive and GPK negative according to the results of absorption of heterophile antibodies in human serum by homogenization with guinea pig kidney (GPK). GPK-positive heterophilic antibodies are present in many infectious diseases and in certain ill-defined diseases such as Chediak-Higashi syndrome (C-H syndrome), Kawasaki disease and some malignant tumors. To directly demonstrate the GPK homogenate interaction with these antibodies, the authors used a mixed agglutination (MA) test, that is, take 100 microliters GPK suspension coated plastic plate wells, and then added 100 microliters of serum dilution, 20 ℃ insulation 2 After three hours, wash with phosphate buffer three times. Add 100 μl trypsinized 0.1% (v / v) sheep or bovine red blood cells to each well and incubate at 20 ° C