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目的建立适合伽玛刀照射的猫功能区慢性点燃癫痫模型。方法雄性成年猫18只,随机分成正常对照组、癫痫模型组、伽玛刀照射组,每组6只。正常对照组猫额功能区皮质注射生理盐水;癫痫模型组猫额功能区皮质多次注射青霉素制作点燃癫痫模型,伽玛刀照射组在造模成功后给予伽玛刀照射。观察各组动物的行为学和脑电图;正常对照组、癫痫模型组两组动物12 w后处死,分光光度计测定额叶皮质功能区的γ-氨基丁酸(γ-GABA)和谷氨酸(Glu)含量。结果正常对照组猫无抽搐发作;癫痫模型组、伽玛刀照射组均出现了抽搐发作,达到了点燃标准;正常对照组皮层脑电图以低波幅快波为主,未见棘、尖波。其余两组皮层脑电图在12 w时均可检测出单发或呈簇状的棘、尖波或多棘波放电。与正常对照组比较,癫痫模型组γ-GABA含量降低,而Glu含量增加,差显著异;伽玛刀照射组可以顺利完成伽玛刀照射全过程。结论该方法能够建立稳定的猫功能区慢性点燃癫痫模型,可用于伽玛刀照射的实验研究,伽玛刀照射治疗癫痫的机制可能与其改善脑功能区皮质Glu和γ-GABA含量有关。
Objective To establish a chronic ignition epilepsy model of cat functional zone suitable for gamma knife irradiation. Methods 18 male adult cats were randomly divided into normal control group, epilepsy model group and gamma knife irradiation group, with 6 in each group. Normal control group, the amount of functional cortical area was injected with normal saline; epilepsy model group, the amount of functional cortical area of penicillin was injected into penicillin to make ignition epilepsy model. Gamma knife irradiation group was given gamma knife irradiation after successful modeling. The behavior and EEG of each group were observed. The animals of both normal control group and epilepsy model group were sacrificed 12 weeks later. The γ-aminobutyric acid (γ-GABA) and glutamate Acid (Glu) content. Results There was no seizure in the normal control group. In the epilepsy model group and the Gamma Knife irradiation group, convulsive seizures occurred and reached the ignition standard. In the normal control group, the cortical EEG was dominated by low-amplitude fast waves without spikes and sharp waves . The remaining two groups of cortical EEG can be detected at 12 w solitary or cluster-like spikes, sharp waves or spike wave discharge. Compared with the normal control group, the content of γ-GABA in the epilepsy model group decreased, while the content of Glu increased significantly, the difference was significant; the gamma knife irradiation group could successfully complete the whole process of gamma knife irradiation. Conclusions This method can establish a stable model of chronic ignition of epilepsy in cats and can be used in the experimental study of Gamma Knife irradiation. The mechanism of Gamma Knife irradiation in the treatment of epilepsy may be related to the improvement of Glu and γ-GABA levels in brain cortex.