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目的探讨泡球蚴囊液对大鼠肝脏星状细胞(HSC-T6)增殖的影响及机制。方法采用不同浓度(0.00~0.90 mg/m L)泡球蚴囊液干预HSC-T6细胞24 h后,在倒置显微镜观察HSC-T6细胞形态变化,利用CCK-8法检测并绘制HSC-T6细胞的生长曲线,采用流式细胞仪检测并分析HSC-T6细胞在不同浓度的泡球蚴囊液培养条件下细胞周期的改变。结果 1 HSC-T6细胞在不同浓度(0.00~0.90 mg/m L)泡球蚴囊液干预24 h后,部分HSC-T6细胞收缩成长梭形,细胞生出许多细长的伪足。2当泡球蚴囊液浓度<0.05 mg/m L时,囊液干预对细胞增殖活性无明显影响(P>0.05),而当泡球蚴囊液浓度>0.05 mg/m L时,随着作用浓度的增加,细胞增殖活性显著增强(P<0.05)。3流式细胞仪检测结果显示,在上述浓度的泡球蚴囊液干预下,处于静止期/DNA合成前期(G0/G1期)的HSC-T6细胞减少(P<0.05),处于DNA合成期(S期)的HSC-T6细胞数量增加(P<0.05),而处于DNA合成后期、细胞分裂期(G2/M期)的细胞数量显著增多(P<0.05)。结论泡球蚴囊液对体外培养大鼠肝脏星状细胞增殖具有浓度依赖的促进作用,其具体机制可能涉及细胞周期调控。
Objective To investigate the effects of cysticercus fluid on the proliferation of rat hepatic stellate cells (HSC-T6) and its mechanism. Methods HSC-T6 cells were treated with cystic fluid of different concentrations (0.00-0.90 mg / mL) for 24 h. The morphological changes of HSC-T6 cells were observed under an inverted microscope. The expression of HSC-T6 cells was detected by CCK-8 assay The growth curve of HSC-T6 cells was detected by flow cytometry and the changes of cell cycle of HSC-T6 cells under different concentrations of Cysticercus cellulosae were studied. Results HSC-T6 cells shrinks and grows fusiform in HSC-T6 cells after cystic fluid of different concentration (0.00 ~ 0.90 mg / mL) for 24 h. Many elongated pseudopods develop. When cystic solution of cysticercus is less than 0.05 mg / m L, cyst fluid has no significant effect on cell proliferation (P> 0.05), while cystic fluid of cystic solution> 0.05 mg / m L, With the increase of concentration, the cell proliferation activity was significantly increased (P <0.05). The results of flow cytometry showed that HSC-T6 cells in the stationary phase / pre-DNA synthesis phase (G0 / G1 phase) were decreased (P <0.05) under the condition of cystic fluid of cystic fluid above the concentration, (P <0.05). However, the number of HSC-T6 cells in S phase increased significantly (P <0.05) at the late stage of DNA synthesis. Conclusion Cysticercus cellulosae fluid can promote the proliferation of hepatic stellate cells in vitro in a concentration-dependent manner. The specific mechanism may involve in the regulation of cell cycle.