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目的:研究扇贝多肽(PCF)对中波紫外线(UVB)所致体外培养人真皮成纤维细胞的凋亡和DNA损伤的影响。方法:用噻唑兰(MTT)法检测成纤维细胞的存活率;流式细胞术测定细胞的凋亡率和胞浆内游离[Ca~(2+)]_i;彗星电泳法检测单细胞DNA受损的程度。结果:PCF(0.25％-1％)能显著提高UVB(1.176×10~(-4) J·cm~(-2))辐射后人真皮成纤维细胞的增殖活性,降低UVB所致细胞的凋亡率和死亡率,减轻受损的程度,同时可降低胞浆内游离[Ca~(2+)]_i(和UVB模型组相比,P<0.01).结论:PCF对抗UVB所致的光老化是基于其减轻UVB对DNA的损伤和降低UVB所致细胞的凋亡,因此,PCF能够在起始阶段阻止紫外线所致光老化的发展。
Objective: To study the effect of scallop polypeptide (PCF) on the apoptosis and DNA damage induced by UVB in human dermal fibroblasts. Methods: The survival rate of fibroblasts was determined by MTT assay. The apoptosis rate and intracellular free [Ca ~ (2 +)] _i were measured by flow cytometry. The single cell DNA The extent of damage. Results: PCF (0.25% -1%) significantly enhanced the proliferation of human dermal fibroblasts induced by UVB (1.176 × 10 -4 J · cm -2), and decreased the apoptosis of cells induced by UVB (P <0.01 compared with UVB model group) .Conclusion: PCF can resist the light induced by UVB [Ca ~ (2 +)] _i Aging is based on its ability to reduce the damage of DNA by UVB and reduce the apoptosis of cells caused by UVB. Therefore, PCF can prevent the development of UV-induced photo-aging in the initial stage.