论文部分内容阅读
背景与目的:甲状腺乳头状癌是预后较好的一种恶性肿瘤,颈部淋巴结转移是其主要的转移方式。近年来迅猛发展起来的基因芯片技术是研究肿瘤生物学行为的一种快速、高效的方法。本研究拟比较初治有颈部淋巴结转移(cN1)的甲状腺乳头状癌组织与正常甲状腺组织的基因差异表达,筛选甲状腺乳头状癌的转移相关基因。方法:分别用Cy3和Cy5两种荧光染料标记两组组织的总mRNA,与含有14112个基因位点的芯片进行杂交。通过对荧光信号的扫描、分析,筛选两组差异表达的基因。结果:共有1212个基因位点在两组间出现差异表达,占总基因点位数的8.71%。其中22个位点呈显著差异表达(上调或下调8倍以上)。在显著下调的6个位点中,有2个位点代表同一个基因序列(NM-001920)——decorin蛋白的mRNA序列。结论:基因芯片是研究疾病发生发展过程中基因表达改变的有效方法之一。PTC的发生发展(包括颈部淋巴结转移)涉及众多的基因参与。Decorin蛋白可能在甲状腺乳头状癌颈部淋巴结转移中发挥重要作用。
BACKGROUND & OBJECTIVE: Papillary thyroid carcinoma is a malignant tumor with good prognosis, and cervical lymph node metastasis is the main mode of metastasis. The rapid development of gene chip technology in recent years is a fast and efficient method to study the biological behavior of tumors. In this study, we compared the gene expression of thyroid papillary carcinoma with normal thyroid tissue in patients with primary cervical lymph node metastasis (cN1), and screened the metastasis-associated genes of thyroid papillary carcinoma. Methods: The total mRNA of two groups of tissues was labeled with Cy3 and Cy5 fluorescent dyes, respectively, and then hybridized with 14112 loci. Through the fluorescence signal scanning, analysis, screening two differentially expressed genes. RESULTS: A total of 1212 loci were differentially expressed between the two groups, accounting for 8.71% of the total loci. 22 of them were significantly differentially expressed (up-regulated or down-regulated more than 8-fold). Two of the six significantly down-regulated genes represent the mRNA sequence of the same gene sequence (NM-001920) -the decorin protein. Conclusion: Gene chip is one of the effective methods to study the gene expression change during the development of disease. The development of PTC (including cervical lymph node metastasis) involving a large number of genes involved. Decorin protein may play an important role in cervical lymph node metastasis of papillary thyroid carcinoma.