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选黑穗病抗性不同的3个谷子品种,研究致病菌黑粉菌对谷田土壤酶活性的影响,分析土壤酶活与植株抗病性的关系。研究发现,谷种拌病菌孢子播种后,3个谷子品种表现不同,冀谷20发病率0.3%,晋谷21发病率10.2%,长农35病穗发生率65.0%;在植株发育的拔节期和抽穗期检测根周土壤酶活性,拌菌组土样中过氧化氢酶(CAT)、多酚氧化酶(PPO)、蔗糖酶、碱性磷酸酶和脲酶活性增高,纤维素酶活性降低,说明病菌能诱导谷田CAT、PPO、蔗糖酶、碱性磷酸酶和脲酶活性增高,且CAT、PPO、蔗糖酶和碱性磷酸酶活性水平在不同品种间存在差异,但脲酶活性差异出现在不同发育期之间,并无品种间差异。结果表明,黑粉菌进入土壤中可影响谷田酶活性,改变土壤微生态,土壤酶参与谷子植株对黑穗病的抗病防御生理过程,不同品种对土壤酶活性有较大影响。
Three millet cultivars with different resistance to smut were selected to study the effects of pathogenic fungus Aeruginosa spp. On soil enzyme activity in the field and the relationship between soil enzyme activity and plant disease resistance. The results showed that the three millet cultivars showed different performance after spore inoculation. The incidence of Ji valley 20 was 0.3%, the incidence of Jingu 21 was 10.2%, and the incidence of panicle 35 was 65.0%. At the jointing stage (P <0.05), the activities of CAT, CAT, PPO, invertase, alkaline phosphatase and urease in the soil samples were increased and the cellulase activity was decreased, The results showed that the pathogen could induce the increase of CAT, PPO, sucrase, alkaline phosphatase and urease activities in the field and CAT, PPO, sucrase and alkaline phosphatase activity in different cultivars, but the difference in urease activity appeared in different development Between the period, there is no difference between varieties. The results showed that the black fungus enter the soil can affect the enzyme activity in the valley, changing soil micro-ecology, soil enzymes involved millet plant disease-resistant defensive physiological processes, different varieties have a greater impact on soil enzyme activity.