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用周龄在25～35Wistar大鼠的头盖骨，经酶消化处理后，在10％胎牛血清的F－12培养液中培养一周，分别加入地塞米松（Dex）、17β-雌二醇（E2）和新型抗骨质疏松药物XW630，利用相差显微镜动态观察成骨细胞（AOB）骨形成情况，连续观察14～20d，经VonKossa染色，用BioMultiScaner－400（BMS－400ToYoSokki）测定骨结节表面积，作为骨形成定量指标，并作统计学分析，结果表明Dex，XW360有促骨形成作用，E2无促骨形成作用。
Were cultured in F-12 medium containing 10% fetal bovine serum for one week with 25 ~ 35 Wistar rat skull after enzymatic digestion. Dexamethasone (Dex), 17β-estradiol (E2) ) And the new anti-osteoporosis drug XW630. Osteoblasts (AOB) bone formation was observed dynamically by phase-contrast microscopy and observed continuously for 14-20 days. Von Kossa staining was used to determine the bone nodule surface area with BioMultiScaner-400 (BMS-400ToYoSokki) As a quantitative index of bone formation, and for statistical analysis, the results showed that Dex, XW360 promote bone formation, E2 did not promote bone formation.