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对餐厨废弃物发酵产黄腐酸分离纯化的工艺条件进行研究,优化离子交换法的工艺参数,以获得高纯度的发酵产黄腐酸。利用单因素试验,采用H+型和NH4+型阳离子交换树脂对餐厨废弃物发酵产黄腐酸进行分离纯化,通过比较2种树脂对黄腐酸粗提液静态交换吸附的效果,筛选出用于黄腐酸溶液纯化的较适合的阳离子交换树脂类型;并利用三因素(上样流速、层析高径比、上样浓度)三水平的正交试验,对筛选出的阳离子交换树脂的纯化工艺参数进行了优化。结果表明:采用离子交换法纯化餐厨废弃物发酵产黄腐酸的最佳工艺条件是采用NH4+型阳离子交换树脂,柱高径比5∶1,上样流速1 BV/h,上样浓度为Na+初始浓度≤700 mg/L(相当于原料0.1 g/mL)。利用该工艺对黄腐酸粗提液进行分离纯化,除杂效果最好,Na+去除率为95.90%,黄腐酸含量可达到82.10%。
The conditions for the separation and purification of fermented fulvic acid from meal waste were studied, and the technological parameters of ion exchange method were optimized to obtain high purity ferulic acid. The single factor experiment was used to separate and purify fulvic acid from the waste of food waste through H + -type and NH4 + -type cation exchange resin. By comparing the static exchanging adsorption of two resins on the crude extract of fulvic acid, Fulvic acid solution purification of the more suitable type of cation exchange resin; and the use of three factors (sample flow rate, chromatographic aspect ratio, sample concentration) three levels of orthogonal test, the selected cation exchange resin purification process The parameters are optimized. The results showed that the optimum conditions for the fermentation of fulvic acid by ion exchange method were NH4 + cation exchange resin, the column height-diameter ratio of 5:1, the flow rate of 1 BV / h, the sample concentration of Na + initial concentration ≤ 700 mg / L (equivalent to 0.1 g / mL of feedstock). The extraction and purification of fulvic acid crude extract by this process has the best removal efficiency, the removal rate of Na + is 95.90% and the content of fulvic acid is up to 82.10%.