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本实验共用80-100克Wistar品系雄性大白鼠60只。实验动物先分为两大组:造型组胃饲1％乙基硫氨酸(D-Ethionine)水溶液11天(胃饲量为每天0.5ml/100gB.W.,下同);对照组胃饲水。实验第12天,随机取部分动物颈椎脱臼处死,检查造型情况。第12天起,造型组动物再分为三组:治疗组停乙基硫氨酸,胃饲0.5g/ml大黄水浸煎剂;自然恢复组停乙基硫氨酸,胃饲水;长乙基硫氨酸组仍胃饲乙基硫氨酸。同期,对照组仍胃饲水。8天后(实验第十九天)处死全部动物。动物处死后观察胰腺破坏情况;取肝、胰组织用CO_2固定,冰冻切片,做琥珀酸脱氢酶、脂酶、单胺氧化酶、中性ATP酶及RNA-DNA的组织化学观察。
In this experiment, 60 male mice weighing 80-100 g Wistar strains were used. The experimental animals were divided into two groups: the styling group was fed stomach with 1% D-Ethionine solution for 11 days (gastric feeding amount was 0.5ml/100gB.W. per day, the same below); the control group was given stomach feeding. water. On the 12th day of the experiment, some animals were randomized to be dislocated and examined for styling. From the 12th day onwards, animals in the modeling group were further divided into three groups: the treatment group was stopped with ethylthione, the stomach was fed with 0.5 g/ml rhubarb water decoction, the natural recovery group was stopped with ethylthione, and the stomach fed water; The ethylthiamidine group was still stomach fed with ethylthione. During the same period, the control group still fed stomach water. All animals were sacrificed 8 days later (test day 19). After the animals were sacrificed, the destruction of the pancreas was observed. The liver and pancreas tissues were fixed with CO 2 and frozen for sectioning. The histochemical observations of succinate dehydrogenase, lipase, monoamine oxidase, neutral ATPase, and RNA-DNA were performed.