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报道了不同来源的野生或栽培的东当归的5S-rDNA基因间隔区序列,并比较了伞形科一些其它药用植物的间隔区。 实验采用野生和栽培品种Angelica acutiloba var.iwatensis和A. acutiloba var.acutiloba,以及栽培的柴胡、荨麻和沙参。从鲜叶中分离出总DNA作为模板,通过PCR进行间隔区域的扩增。从所搜集植物的5S-rRNA序列中设计出PCR的引物。PCR混合物包括5.0nmol的dNTP,各20pmol的引物,20ng的模板DNA和1单位的Teh DNA聚合酶,置于25μl PCR缓冲液中。进
The 5S-rDNA gene spacer sequences of wild or cultivated DongDong of different origins were reported, and the spacers of some other medicinal plants of Umbelliferae were compared. Wild and cultivars Angelica acutiloba var.iwatensis and A. acutiloba var. acutiloba were used in the experiments, as well as cultivation of Bupleurum, Ricinus communis and Radix Glehniae. Total DNA was isolated from fresh leaves as a template, and the gap region was amplified by PCR. PCR primers were designed from the 5S-rRNA sequences of the collected plants. The PCR mixture consisted of 5.0 nmol of dNTPs, 20 pmol of each primer, 20 ng of template DNA, and 1 unit of Teh DNA polymerase in 25 l of PCR buffer. Into