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用逆转录－聚合酶链反应法（RT—PCR）检测了原发性肝癌（PLC）患者血清及肝癌和癌旁肝组织中的庚型肝炎病毒（HGV）RNA，以PCR－双脱氧末端终止法测定了PCR产物的核苷酸序列。结果显示，血清和肝组织中HGVRNA的检出率分别为19．4％（13／67）和25．7％（9／35），且HGVRNA在肝癌组织和癌旁肝组织中同时存在；血清和肝组织中HGVRNA检测结果的符合率为85％；5非编码区（5＇－NCR）扩增片段的核着酸序列与美国株GBV—C的同源性为91．7％。本研究结果提示HGV感染在我国PLC患者中存在，且血清HGVRNA的检测能反映HGV在肝内的复制情况。
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the hepatic hepatitis virus (HGV) RNA in serum, liver cancer, and adjacent liver tissues of patients with primary liver cancer (PLC) and terminated by PCR-dideoxy end termination. The nucleotide sequence of the PCR product was determined. The results showed that the detection rates of HGVRNA in serum and liver tissues were 19.4% (13/67) and 25.7% (9/35), respectively, and HGVRNA existed in both hepatocellular carcinoma and adjacent liver tissue; The coincidence rate of HGVRNA detection in liver tissue was 85%. The nucleotide sequence of the 5′-NCR amplified fragment was 91.7% homologous to the American strain GBV-C. The results of this study suggest that HGV infection exists in PLC patients in China, and the detection of serum HGVRNA can reflect the replication of HGV in the liver.