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目的探讨硫酸镁对大鼠放射性脑损伤神经功能及即早基因表达的影响。方法将SD大鼠随机分为对照组(n=26)、照射组(n=32)和照射加药组(n=32),用5 MeV电子线进行单次半脑照射20Gy建立放射性脑损伤模型。在照后2h、24h用免疫荧光组织化学技术观察海马结构c-fos基因表达并计数阳性细胞,在照后第1w、4w、8w行Y-型迷宫测试观察学习和记忆能力,并进行病理形态学观察。结果与对照组相比,照射组大鼠在2h、24h时海马结构c-fos基因表达明显增加(P<0.01),Y-迷宫学习和记忆成绩明显降低(P<0.01);与照射组相比,照射加药组Fos阳性细胞数明显减少(P<0.05),学习尝试次数在第4周、8周较前者减少(P<0.01),记忆再现次数增加(P<0.05);病理形态学上照射加药组亦比照射组损伤减轻。结论硫酸镁对大鼠放射性脑损伤所致的早期学习记忆功能降低有改善作用,对神经细胞有保护作用,其下调海马结构神经细胞Fos蛋白的表达可能是硫酸镁减轻细胞损伤作用机制之一。
Objective To investigate the effects of magnesium sulfate on neurological function and immediate early gene expression in radiation-induced brain injury in rats. Methods Sprague-Dawley rats were randomly divided into control group (n = 26), irradiation group (n = 32) and irradiation plus drug group (n = 32) model. The expression of c-fos gene in hippocampal formation was observed by immunofluorescence histochemistry at 2h and 24h after irradiation, and the positive cells were counted. The learning and memory abilities of Y-type maze test were observed at 1w, 4w and 8w after irradiation and the pathological changes were observed Learn to observe. Results Compared with the control group, the expression of c-fos gene in hippocampal formation was significantly increased (P <0.01) and the Y-maze learning and memory scores were significantly decreased (P <0.01) (P <0.05). The number of learning attempts in the fourth week and the eighth week decreased compared with the former (P <0.01) and the frequency of memory regeneration increased (P <0.05). Pathological morphology On the irradiation plus drug group than the irradiation group lessened. Conclusion Magnesium sulfate can improve the learning and memory abilities of early brain injury induced by radioactive brain injury in rats and protect neurons. Down-regulation of Fos protein in hippocampal neurons may be one of the mechanisms of magnesium sulfate in reducing cell injury.