【摘 要】
：
目的 检测多发性骨髓瘤(MM)患者Th9、Thl7和调节性T细胞及白介素-9(IL-9)、白介素-17(IL-17)、转化生长因子-β(TGF-β)的异常表达并探讨其在MM初诊中的意义.方法 运用流式细胞仪检测54名MM患者(病例组)与45名健康体检者(对照组)外周血Th9、Thl7、Treg细胞水平,2组外周血中IL-9、IL-17、TGF-β3等细胞因子的浓度用酶联免疫吸附法测定.结果 病例组外周血Th9、Th17细胞比例较对照组均显著升高[(1.37±0.39)％ vs.(0.79±0.26)％,P
【机 构】
：
大连市血液中心,辽宁大连116001
论文部分内容阅读
目的 检测多发性骨髓瘤(MM)患者Th9、Thl7和调节性T细胞及白介素-9(IL-9)、白介素-17(IL-17)、转化生长因子-β(TGF-β)的异常表达并探讨其在MM初诊中的意义.方法 运用流式细胞仪检测54名MM患者(病例组)与45名健康体检者(对照组)外周血Th9、Thl7、Treg细胞水平,2组外周血中IL-9、IL-17、TGF-β3等细胞因子的浓度用酶联免疫吸附法测定.结果 病例组外周血Th9、Th17细胞比例较对照组均显著升高[(1.37±0.39)％ vs.(0.79±0.26)％,P＜0.05;(2.02±0.41)％ vs.(1.18±0.32)％,P＜0.05],CD4+ CD25+ FoxP3+ Treg细胞比例较对照组显著降低[(4.92±0.83)％ vs.(7.04±1.85)％,P＜0.05],病例组外周血中细胞因子IL-9、IL-17的表达水平较对照组均显著升高[(25.74±7.33)％ vs.(16.82±5.58)％,P＜0.05;(11.01±3.71)％ vs.(7.68±2.57),P＜0.05],TGF-β的表达水平较对照组显著降低[(3.73±1.44)％ vs.(6.95±2.12)％,P＜0.05].结论 MM患者外周血Th9、Thl7、Treg细胞比例异常,伴有细胞因子IL-9、IL-17、TGF-β表达异常,提示可能在MM初诊中起到重要作用.
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