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目的探讨黄芩苷对海人酸诱导的小鼠癫痫持续状态后海马组织白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的影响。方法将54只ICR雄性小鼠随机分为对照组、癫痫持续状态(SE)组、黄芩苷治疗组,每组18只。采用侧脑室注入海人酸建立小鼠癫痫持续状态模型。HE染色观察黄芩苷对小鼠癫痫持续状态后海马神经细胞的形态学影响。通过RT-PCR和Western blot分别检测小鼠海马组织中IL-1βmRNA、TNF-αmRNA及IL-1β、TNF-α蛋白的表达量。结果黄芩苷明显改善了SE后小鼠海马组织的病理形态学,并且可降低IL-1β、TNF-α的表达(P<0.05)。结论黄芩苷可能通过降低癫痫鼠海马组织中IL-1β、TNF-α的表达发挥抗炎作用,从而对脑组织进行保护。
Objective To investigate the effects of baicalin on the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the hippocampus of hippocampus after the status of epilepsy induced by kainate in mice. Methods 54 ICR male mice were randomly divided into control group, SE group, baicalin treatment group, 18 rats in each group. The intracerebroventricular injection of kainic acid was used to establish the model of status epilepticus in mice. The Morphological Changes of Hippocampal Neurons Treated with Baicalin after Persistent Epilepsy in Mice Observed by HE Staining. The expression of IL-1βmRNA, TNF-αmRNA, IL-1βand TNF-αprotein in hippocampus were detected by RT-PCR and Western blot respectively. Results Baicalin significantly improved the histopathology of hippocampus in SE mice and decreased the expression of IL-1β and TNF-α (P <0.05). Conclusion Baicalin may play an anti-inflammatory role by decreasing the expression of IL-1β and TNF-α in the hippocampus of epileptic rats, thus protecting brain tissue.