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清醒大鼠置于一循环水加热装置中,经45℃热应激致肛温达42℃时,维持不同时间,断头活杀,取血及肺,经处理后用高效液相-电化学检测器(HPLC-DE)检测方法,使用国产 C_(18)反相填料,测定去甲肾上腺素(NE),肾上腺素(E)的含量。结果表明,NE 在血浆和肺中的正常值分别为4.8±1.2ng/ml,151.4±33.7ng/g 湿重;E 的正常值分别为5.0±1.5ng/ml,11.0±2.0ng/g 湿重。热应激后血和肺中 NE 降低,而 E 明显升高。大鼠经热应激后回到室温下恢复4h 后,血和肺中 NE 回到正常水平,而 E 的含量仍维持在一高水平。
Awake rats were placed in a circulating water heating device, the 45 ℃ heat stress caused rectal temperature up to 42 ℃, to maintain a different time, decapitated live kill, take blood and lungs, after treatment with high performance liquid-electrochemical The content of norepinephrine (NE) and epinephrine (E) was determined by HPLC-DE method using domestic C 18 reversed phase packing. The results showed that the normal values of NE in plasma and lung were 4.8 ± 1.2ng / ml and 151.4 ± 33.7ng / g wet weight, respectively. The normal values of E were 5.0 ± 1.5ng / ml and 11.0 ± 2.0ng / g respectively weight. After heat stress, the blood and lung NE decreased, while E increased significantly. Rats returned to normal temperature by heat stress and returned to normal temperature after returning to room temperature for 4 hours, while the content of E remained at a high level.