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目的 探讨低频电针对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠卵巢组织中Bax、Bcl-2表达的影响.方法 24只3周龄雌性SD大鼠等量随机分成模型组(M组)、针刺组(A组)与正常对照组(C组),每组8只.通过注射睾酮和高脂饲料喂养进行造模.从1 1周龄开始,A组连续5周,每周5次,每次30 min低频电针,M组和C组不干预.在治疗前后,空腹取血检测睾酮(T)、胰岛素(FINS)、血糖(FBG),比较胰岛素抵抗指数(HOMA-IR).卵巢组织通过HE染色观察结构,采用荧光定量PCR及Western blot检测卵巢组织中Bax、Bcl-2 mRNA及蛋白表达,并计算Bax/Bcl-2比值.结果 自8周龄起,M组体重均重于C组(P ＜0.05,P＜0.01);自14周龄起,A组体重明显低于M组(P＜0.05).1 1周及16周,M组血清T、FINS及HOMA-IR水平较C组显著升高(P＜0.01);16周龄A组血清T、FINS及HOMA-IR水平较M组及治疗前明显降低(P ＜0.05,P＜0.01).A组卵巢切片HE染色可见多个黄体存在,囊性卵泡减少,颗粒细胞层比M组明显增厚;M组Bax蛋白及mRNA表达高于C组,Bcl-2蛋白及mRNA表达低于C组(P ＜0.05,P＜0.01);A组Bax蛋白及mRNA表达低于M组(P＜0.05),Bax/Bcl-2比值低于M组(P＜0.01).结论 低频电针可能通过降调卵巢组织中Bax mRNA及蛋白表达和降低Bax/Bcl-2比值,抑制颗粒细胞凋亡,改善卵巢局部微环境,促进黄体生成,恢复正常排卵周期.“,”Objective To observe the effects of low frequency electroacupuncture (LF-EA) on the expressions of Bcl-2 associated X protein (Bax),B-cell lymphoma-2 (Bcl-2) in the ovary tissue of polycystic ovary syndrome (PCOS) rats.Methods Totally 24 female 3 weeks old Sprague-Dawley (SD)rats were randomly divided into three groups,the normal control group(C),the model group (M),and the EA group(A),8 in each group.Rats in the M group and the A group were fed with high-fat diet and injected with andronate for 8 weeks.Rats in the A group received LF-EA for 5 successive weeks,30 min each time 5 times per week from 11 weeks old.Rats in the M group and the C group received no intervention.Levels of testosterone (T),fasting insulin (FINS),and fasting blood glucose (FBG) were detected before and after intervention.HOMA-IR was compared.The structure of the ovarian tissue was observed by HE staining.mRNA and protein expressions of Bax and Bcl-2 were measured via real-time fluorescent quantitative PCR and Western blot.Bax/Bcl-2 of the ovarian tissue was calculated.Results The weight of the M group was heavier than that of the C group from week 8 (P ＜0.05,P ＜0.01).The weight of the A group was lighter than that of the M group from week 14 (P ＜0.05).The levels of serum T and FINS,as well as HOMA-IR significantly increased in the M group than those of the C group at week 11 and week 16 (P ＜0.01).The levels of serum T and FINS,as well as HOMA-IR significantly decreased in the A group than those of the M group at week 16 (P ＜0.05,P ＜0.01).HE staining of the ovarian tissue showed some corpora lutea existed in the A group,with lesser cystic follicles.The granular cell layer was obviously thicker in the A group than in the M group.mRNA and protein expressions of Bax were higher in the M group than in the C group;those of Bcl-2 were lower in the M group than in the C group (P ＜0.05,P ＜0.01).mRNA and protein expressions of Bax were lower in the A group than in the M group (P ＜0.05);Bax/Bcl-2 ratio was lower in the A group than in the M group (P ＜0.01).Conclusion LF-EA might inhibit apoptosis of ovarian granulosa cells,improve local ovarian miroenvironment,promote luteal production,and restore normal ovulatory cycle possibly by down-regulating mRNA and protein expressions of Bax and decreasing Bax/Bcl-2 ratio.