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目的：测定消旋吡喹酮（Ｐｒａ）、左旋吡喹酮（Ｌ－Ｐｒａ）和右旋吡喹酮（Ｄ－Ｐｒａ）对不同发育期日本血吸虫的作用。方法：用含２０％小牛血清的ＰＲＭＩ１６４０培养不同发育期的血吸虫，测定上述三种药物的体外抗血吸虫作用。体内试验系于小鼠感染血吸虫尾蚴后不同时间灌服（ｉｇ）Ｐｒａ、Ｌ－Ｐｒａ或Ｄ－Ｐｒａ，根据残留平均虫数评估药效。结果：依据药物引起虫的皮层损害程度，虫龄为２８ｄ（ｄ２８）和３５ｄ（ｄ３５）的成虫对Ｌ－Ｐｒａ最敏感，而１４ｄ（ｄ１４）童虫则最不敏感。在药物浓度为０．１－１μｇ／ｍｌ时，Ｌ－Ｐｒａ的抗血吸虫作用较Ｐｒａ的为强，即使Ｌ－Ｐｒａ的浓度减至Ｐｒａ最低有效药浓度的１／２亦有效。在体外，上述浓度的Ｄ－Ｐｒａ对不同发育期血吸虫无明显作用。感染小鼠ｉｇ单剂量的Ｌ－Ｐｒａ，Ｐｒａ或Ｄ－Ｐｒａ３００ｍｇ／ｋｇ或５００ｍｇ／ｋｇ，仅前２种药物对３ｈ（ｄｏ）、２１ｄ（ｄ２１）童虫和ｄ２８及ｄ３５成虫有较明显的疗效，而对３ｄ（ｄ３）、７ｄ（ｄ７）和ｄ１４童虫的疗效甚差或无效。与Ｌ－Ｐｒａ和Ｐｒａ相比，Ｄ－Ｐｒａ仅对ｄ３５成虫有较差的疗效。感染ｄ３５血吸虫成虫的小鼠用Ｌ－Ｐｒａ１５０ｍｇ／ｋｇｉｇ治疗，其疗效与用?
Objective: To determine the effect of praziquantel (Pra), L-Pra and D-Pra on Schistosoma japonicum at different developmental stages. Methods: Schistosoma japonicum was cultured in PRMI1640 containing 20% calf serum at different developmental stages to determine the in vitro anti-schistosome effect of these three drugs. In vivo tests were performed on mice infected with Schistosoma japonicum cercariae at different times (ig) Pra, L-Pra or D-Pra, according to the residual average number of insects to assess efficacy. Results: According to the degree of cortical damage caused by the drug, adult worms 28 d (d28) and 35 d (d35) were the most sensitive to L-Pra, while insects 14 d (d14) were the most insensitive. The anti-schistosome effect of L-Pra was stronger than that of Pra at the drug concentration of 0.1-1 μg / ml, which was effective even when the concentration of L-Pra was reduced to 1/2 of the minimum effective dose of Pra. In vitro, the above concentration of D-Pra had no significant effect on schistosomiasis in different developmental stages. Infection with single doses of L-Pra, Pra or D-Pra at 300 mg / kg or 500 mg / kg in mice ig, only the first two drugs have a significant effect on 3h (do), 21d (d21) and adult d28 and d35 , While the effects on 3d (d3), 7d (d7) and d14 squirrels were poor or ineffective. Compared to L-Pra and Pra, D-Pra has poorer efficacy on d35 adults only. Mice infected with d35 schistosome adult with L-Pra150mg / kgig treatment, its efficacy and use?