论文部分内容阅读
植物体内普遍存在内生真菌,它们可以产生与宿主相同或相似的生理活性物质。通过微生物发酵产生生理活性物质可以为解决能源短缺和寻找替代能源开辟一条新途径。作者初步探讨了分离和纯化高产菌株以及对其发酵代谢产物的结构鉴定的方法。采用溶剂提取法、薄层色谱法和柱色普法,对从东北红豆杉(T.cuspidataSieb.etZucc.)上分离筛选出的高产紫杉烷类物质紫杉链格孢(Alternariaalternatavar.taxi1011Y.XiangetLUAn-guo)1011菌株的发酵产物进行分离纯化,提取了一个化合物Ⅰ。经紫外扫描、红外扫描、质谱、核磁共振等方法鉴定,确定该化合物为紫杉烷类二萜Ⅲ型化合物。图1表2参12。
Endophytic fungi are ubiquitous in plants and they produce the same or similar physiologically active substances as the host. Producing bioactive substances through microbial fermentation can open up a new way to solve the energy shortage and find alternative energy sources. The authors initially explored the methods for the isolation and purification of high-yielding strains and their structural identification of their fermented metabolites. Solvent extraction, thin layer chromatography and column chromatography were used to detect the taxane of Alternaria alternata var. Taxi1011Y.XiangetLUAn- guo) 1011 strain was isolated and purified, and a compound Ⅰ was extracted. The compounds were identified as taxane-type diterpene type III by UV scanning, IR, MS and NMR. Figure 1 Table 2 Reference 12.