论文部分内容阅读
目的探讨开腹及不同气腹环境对直肠癌COLO-320细胞侵袭能力的影响。方法 64只裸鼠均分为开腹(O)组、CO2气腹(A)组、N2气腹(B)组和无气腹腹腔镜(LC)组。术前30 min裸鼠腹腔内注入1×107直肠癌COLO-320细胞。模拟开腹手术及相应腹腔镜手术时的60 min气腹环境。3周后每组取10只裸鼠处死,观察腹腔及各脏器的成瘤情况;剩余6只观察生存时间。结果裸鼠致瘤率:O组,100%(10/10);A组,100%(10/10);B组,90%(9/10);LC组,90%(9/10)(P>0.05);LC组腹腔内瘤结节数和不同脏器成瘤率最低(P<0.05),其余三组间相仿(P>0.05)。四组裸鼠生存时间差异无统计学意义(P>0.05)。结论与开腹手术相比,CO2或N2气腹并不增加直肠癌细胞在腹腔内以及对各脏器的侵袭转移能力,对致瘤裸鼠的生存时间无影响;而无气腹腹腔镜在减少肿瘤细胞侵袭及转移有一定的作用。
Objective To investigate the effect of laparotomy and different pneumoperitoneum on invasiveness of rectal cancer COLO-320 cells. Methods 64 nude mice were divided into two groups: open (O), CO2 pneumoperitoneum (A), N2 pneumoperitoneum (B) and no pneumoperitoneum (LC). Nude mice were intraperitoneally injected with 1 × 107 colorectal cancer COLO-320 cells 30 min before operation. Simulate laparotomy and laparoscopic surgery 60 min when the pneumoperitoneum environment. After 3 weeks, 10 nude mice in each group were sacrificed to observe the tumor formation in the abdominal cavity and various organs. The remaining 6 rats were observed for their survival time. Results: The tumorigenic rate in nude mice was 100% (10/10) in group O, 100% (10/10) in group A, 90% (9/10) in group B, 90% (9/10) in group LC, (P> 0.05). The number of intra-abdominal tuberculoma in LC group and the tumor formation rate of different organs were the lowest (P <0.05), and the other three groups were similar (P> 0.05). The survival time of four nude mice had no significant difference (P> 0.05). Conclusions Compared with open operation, pneumoperitoneum of CO2 or N2 does not increase the invasion and metastasis of rectal cancer cells in abdominal cavity and various organs, and has no effect on the survival time of tumor-bearing nude mice. However, pneumoperitoneum without pneumoperitoneum Reduce tumor cell invasion and metastasis have a certain effect.